secondary antibody

Mouse Anti-Human IgG4 (pFc')-AP

MBS674071

1 mL

255 USD

Secondary Antibody

Mouse Anti-Human IgG4 (pFc')-AP

Mouse Anti-Human IgG4 (gamma 4 chain specific)

Mouse Anti-Human IgG4 (pFc')- Alkaline Phosphatase-Labeled; Mouse Anti-Human IgG4 (gamma 4 chain specific)

Monoclonal

Mouse (BALB/c)IgG3 kappa

HP6023

Human

Human IgG4 pFc; Mr 146kDa

Alkaline Phosphatase (AP) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Each reagent is stable for the period shown on the bottle label if stored as directed.

Quality Tested: ELISA; FLISA. Reported in literature: FC; IHC-FS; WB; IP; SPR

WORKING DILUTIONS:. ELISA:. AP conjugate: 1:1,000-1:2,0000. HRP conjugate: 1:2,000-1:8,000. BIOT conjugate: 1:5,000-1:10,000. FLISA:. FITC conjugate: 1:200-1:400. AF647 conjugate: <= 5ug/ml. PE conjugate: <= 1ug/ml. Other applications: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

Immunogen: Human IgG4 myeloma protein

Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA for conformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhale, contact a physician immediately. Sodium azide yields toxic hyrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Mouse Anti-Human IgG4 (?4 chain specific)

1 mL

255 USD