catalog number :
MBS674037
products type :
Secondary Antibody
products full name :
Mouse Anti-Human IgG2 (Fc)-AP
products short name :
[Mouse Anti-Human IgG2 (gamma 2 chain specific)]
products name syn :
[Mouse Anti-Human IgG2 (Fc)- Alkaline Phosphatase-Labeled; Mouse Anti-Human IgG2 (gamma 2 chain specific)]
isotype :
Mouse IgG1 kappa
host :
Host: Mouse. Source: Ascites Fluid
specificity :
Reacts with the Fc portion of the heavy chain of human IgG2 as demonstrated by ELISA
form :
Alkaline Phosphatase (AP) Conjugate
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
ELISA; FLISA; Immunoblot; Immunocytochemistry; Immunohistochemistry (Frozen Sections); Western Blot
app notes :
WORKING DILUTIONS:. FLISA: . R-PE conjugate 1ug/mL . AF647 conjugate 1 ug/mL . AF488 conjugate 1:200 - 1:400 . ELISA. Purified antibody < or = 5ug/mL . AP conjugate 1:1,000-1:2,000 . HRP conjugate 1:4,000-1:8,000 . BIOT conjugate 1:5,000-1:20,000 . Other Applications: Sinve applications vary, you should determine the optimm working dilution of the product that is appropriate for your specific need.
other info2 :
Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent.
products categories :
Mouse Anti-Human IgG2 (?2 chain specific)