secondary antibody

Mouse Anti-Human IgG1 (hinge)-AP

MBS674027

1 mL

235 USD

Secondary Antibody

Mouse Anti-Human IgG1 (hinge)-AP

Mouse Anti-Human IgG1 (gamma 1 chain specific)

Mouse Anti-Human IgG1 (hinge)- Alkaline Phosphatase-Labeled; Mouse Anti-Human IgG1 (gamma 1 chain specific)

Monoclonal

Mouse IgG1

4.00E+03

Mouse

Human

Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA or FLISA. May also react with IgG from other species.

Alkaline Phosphatase (AP) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

ELISA; FLISA

WORKING DILUTIONS:. FLISA: . FITC and AF488 conjugates 1:200 - 1:400. R-PE conjugate 1ug/mL. AF647 conjugate 1 ug/mL. ELISA. Purified antibody < or = 5ug/mL. AP conjugate 1:1,000-1:2,000. HRP conjugate 1:2,000-1:8,000. BIOT conjugate 1:5,000-1:10,000. Other Applications: Since applications vary, you should determine the optimm working dilution of the product that is appropriate for your specific need.

Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA or FLISA for comformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium aide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Mouse Anti-Human IgG1 (?1 chain specific)

1. Mark T. Whary, Natliya Sundina, Luis E. Bravo, Pelayo Correa, Francisco Quinones, Fanny caro, and James G. Fox 2005 Cancer Epidemiol Biomarkers Prev 14(6):1464.

1 mL

235 USD