catalog number :
MBS674021
products type :
Secondary Antibody
products full name :
Mouse Anti-Human IgG (Fc)-APC
products short name :
Mouse Anti-Human IgG (Fc) (gamma chain specific)
products name syn :
Mouse Anti-Human IgG (Fc) - Allophycocyanin; Mouse Anti-Human IgG (Fc) (gamma chain specific)
isotype :
Mouse IgG2b kappa
specificity :
Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA; may also react with IgG from other species.
form :
Allophycocyanin (APC) Conjugate
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
ELISA; Immunocytochemistry; Immunohistochemistry (Frozen Sections); Western Blot; Indirect immunofluorescent staining; Fluorescent - Linked- Immunosorbent - assay (FLISA); Dot- and slot-immunoblotting.
app notes :
Research Application(s): Indirect immunofluorescent staining of IgG+ human B lymphocytes. Immunofluorescence Suggested Dilution: <= 0.1 ug/10^6 cells. ELISA. AP conjugate 1:2,000-1:8,000
other info2 :
Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA, FLISA and/or Flow Cytometry for conformance to characteristics of a standard reference reagent.
products categories :
Mouse Anti-Human IgG