secondary antibody

Mouse Anti-Human IgG (Fc)-HRP

MBS674018

1 mL

230 USD

Secondary Antibody

Mouse Anti-Human IgG (Fc)-HRP

Mouse Anti-Human IgG (Fc) (gamma chain specific)

Mouse Anti-Human IgG (Fc) - HRP; Mouse Anti-Human IgG (Fc) (gamma chain specific)

Monoclonal

Mouse IgG2b kappa

H2

Mouse

Human

Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA; may also react with IgG from other species.

Horseradish Peroxidase (HRP) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

ELISA; Immunoblot; Immunocytochemistry; Immunofluorescence; Immunohistochemistry (Frozen Sections); Western Blot

ELISA:. HRP conjugate: 1:4,000-1:8,000. Other applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by ELISA, FLISA, and/or flow cytometry to conform to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Mouse Anti-Human IgG

1 mL

230 USD