secondary antibody

Mouse Anti-Human IgG (Fc)-AP

MBS674011

1 mL

240 USD

Secondary Antibody

Mouse Anti-Human IgG (Fc)-AP

Mouse Anti-Human IgG (gamma chain specific)

Mouse Anti-Human IgG (Fc)- Alkaline Phosphatase-Labeled; Mouse Anti-Human IgG (gamma chain specific)

Monoclonal

Mouse IgG1 kappa

JDC-10

Mouse

Human

Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA, FLISA, and/pr flow cytometry. May also react with IgG from other species.

Alkaline Phosphatase (AP) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Each reagent is stable for the period shown on the bottle label if stored as directed.

Research Application(s): Indirect immunofluorescent staining; ELISA; FLISA; Immunoblotting; Immunohistochemistry (Frozen Section) (IHC-F); Immunocytochemistry (ICC)

WORKING DILUTIONS:. Flow Cytometry:. Purified (UNLB) antibody: < or = 1ug/10^6 cells. BIOT conjugate: < or = 1ug/10^6 cells. PE conjugate: < or = 0.2 ug/10^6 cells. ELISA:. AP conjugate: 1:1,000-1:4,000. HRP conjugate: 1:2,000-1:8,000. BIOT conjugate: 1:5,000-1:10,000. Other applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes, or cloting. Wear eye of face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Mouse Anti-Human IgG

1 mL

240 USD