catalog number :
MBS673765
products type :
Secondary Antibody
products full name :
Mouse Anti-Rat IgM-HRP
products short name :
Mouse Anti-Rat IgM (u chain specific)
products name syn :
Mouse Anti-Rat IgM - HRP; Mouse Anti-Rat IgM (u chain specific)
isotype :
Mouse IgG1 kappa
specificity :
Reacts with the u heavy chain (Fc) of rat IgM
form :
Houseradish Peroxidase (HRP) Conjugate
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
ELISA; Flow Cytometry; Immunofluorescence
app notes :
Research Application(s): Identification and enumeration of IgM+ cells by flow cytometry . Identification and enumeration of IgM+ cells by immunofluorescence microscopy . Second step reagent for rat IgM monoclonal antibodies . ELISA . Surface IgM detection . B cell stimulation . ELISA Suggested Dilution: 1:2,000-1:5,000
other info2 :
Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested as a second step reagent by flow cytometry and/or ELISA to conform with characteristics of a standard reference reagent. Representative data are included in this product insert.
products categories :
Mouse Anti-Rat IgM