antibody

Rat Anti-Mouse CD107a/LAMP-1-UNLB

MBS673717

0.5 mg

290 USD

monoclonal

rat

nonconjugated

1D4B

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

Secondary Antibody

Rat Anti-Mouse CD107a/LAMP-1-UNLB

CD107a (LAMP-1)

Rat Anti-Mouse CD107a/LAMP-1 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD107a (LAMP-1)

Monoclonal

Rat (Sprague-Dawley) IgG2a kappa

1D4B

Rat

Mouse

Murine CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa). CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles.^1 It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion.^2 CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice.^3 LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases.^4

Purified (UNLB) Antibody

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot

Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells

Immunogen: Plasma membrane fraction of mouse embryo NIH3T3 cell line

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.

1. Chen, J.W., W. Pan, M.P. d'Souza and J.T. August. 1985. Arch. Biochem. Biophys. 239:574. 2. Arterburn, L.M., B.J. Earles, and J.T. August. 1990. J. Biol. Chem. 265:7419. 3. Chen, J.W., G.L. Chen, M.P. d'Souza, T.L. Murphy, and J.T. August. 1986. Biochem. Soc. Symp. 51:97. 4. Fukuda, M. 1991. J. Biol. Chem. 266:21327. 6. Rohrer, J., A. Schweizer, D. Russell, and S. Kornfeld. 1996. J. Cell. Biol. 132:565.

0.5 mg

290 USD