Rat Anti-Mouse MHC Class II-UNLB | MyBioSource MBS673637 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse MHC Class II-UNLB

catalog :

MBS673637

quantity :

0.5 mg

price :

300 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

NIMR-4

reactivity :

human, mouse

application :

flow cytometry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS673637

products type :

Secondary Antibody

products full name :

Rat Anti-Mouse MHC Class II-UNLB

products short name :

MHC Class II

products name syn :

Rat Anti-Mouse MHC Class II - Purified, Unlabeled Antibody; Rat Anti-Mouse MHC Class II

other names :

MHC class II; MHC class II HLA-DQ-beta-1; HLA class II histocompatibility antigen, DQ beta 1 chain; MHC DQ beta; MHC class2 antigen; OTTHUMP00000029167; OTTHUMP00000178569; OTTHUMP00000178570; OTTHUMP00000178571; lymphocyte antigen; MHC class II antigen DQB1; MHC class II DQ beta chain; MHC class II antigen HLA-DQ-beta-1; MHC class II HLA-DQ beta glycoprotein; major histocompatibility complex class II beta; HLA class II histocompatibility antigen, DQ beta 1 chain-like; major histocompatibility complex, class II, DQ beta 1

other gene names :

HLA-DQB1; HLA-DQB1; IDDM1; CELIAC1; HLA-DQB

uniprot entry name :

O19707_HUMAN

clonality :

Monoclonal

isotype :

Rat IgG2b

clone :

NIMR-4

host :

Rat

reactivity :

Mouse

sequence length :

229

specificity :

MHC Class II molecules. The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells.

form :

Purified (UNLB) Antibody

storage stability :

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

tested application :

Flow Cytometry

app notes :

Research Application(s): Complement-dependent cytotoxicity assays. Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells

other info2 :

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.

products references :

1. R.M.E. Parkhouse. 1996. Personal communication.

ncbi gi num :

1160938

uniprot acc num :

O19707

ncbi mol weight :

26,489 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83069)

ncbi summary :

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

uniprot summary :

Sequence similarities: Contains 1 Ig-like C1-type (immunoglobulin-like) domain. SAAS SAAS014745

size1 :

0.5 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage