antibody

Rat Anti-Mouse CD104-UNLB

MBS673614

0.5 mg

285 USD

monoclonal

rat

nonconjugated

346-11A

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - frozen section

Antibody

Rat Anti-Mouse CD104-UNLB

CD104 (beta 4 Integrin)

Rat Anti-Mouse CD104 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD104 (beta 4 Integrin)

Monoclonal

Rat (Fischer 344) IgG2a kappa

346-11A

Rat

Mouse

Integrin beta 4 chain (CD104), Mr 200 kDa. CD104 represents the integrin beta 4 subunit that associates non-covalently with integrin alpha 6 (CD49f) to form the alpha 6 beta 4 integrin heterodimer.2, 3 The alpha 6 beta 4 complex is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia. It is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes, and has been found to be associated with a number of malignant tissues.3-5, 6 Hemidesmosomal alpha 6 beta 4 plays an important role in the adhesion of epithelial cells to basement membranes via interactions with laminin and/or kalinin anchoring filaments.7 It is not known if the monoclonal antibody 346-11A can block this adhesion.

Purified (UNLB) Antibody

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot

Immunohistochemistry Suggested Dilution: 1-5 ug/mL

Immunogen: Tumor-associated antigen TSP-180 immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.^1

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by immunohistochemical staining to conform to characteristics of a standard reference reagent.

1. Kennel, S.J., L.J. Foote, and K.M. Flynn. 1986. Cancer Res. 46:707. 2. Kennel, S.J., L.J. Foote, R. Falcioni, A. Sonnenberg, C.D. Stringer, C. Crouse, and M.E. Hemler. 1989. J. Biol. Chem. 264:15515. 3. Kennel, S.J., V. Godfrey, L.Y. Ch'ang, T.K. Lankford, L.J. Foote, and A Makkinje. 1992. J. Cell. Sci. 101:145. 4. Sonnenberg, A., C.J.T. Linders, J.H. Daams, and S.J. Kennel. 1990. J. Cell. Science 96:207. 5. Wadsworth, S., M.J. Halvorson, and J.E. Coligan. 1992. J. Immunol. 149:421. 6. Constantini, R.M., R. Falcioni, P. Battista, G. Zupi, S.J. Kennel, A., Colassante, I. Venturo, C.G. Curcio, and A. Sacchi. 1990. Cancer Res. 50:6107. 7. Sonnenberg, A., J. Calafat, H. Janssen, H. Daams, L.M.H. vander Raaij-Helmer, R. Falcioni, S.J. Kennel, J.D. Aplin, J. Baker, M. Loizdou, and D. Garrod. 1991. J. Cell. Biol. 113:907.

0.5 mg

285 USD