catalog number :
MBS673552
products type :
Secondary Antibody
products full name :
Mouse Anti-Mouse Ly-49F-BIOT
products short name :
Ly49F
products name syn :
Mouse Anti-Mouse Ly-49F - Biotin; Mouse Anti-Mouse Ly49F
isotype :
Mouse (BALB/c) IgG1 kappa
specificity :
B6 allele of the Ly49F receptor. The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains ~ 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice.1
form :
Biotin (BIOT) Conjugate
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
Flow Cytometry
app notes :
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
other info1 :
Immunogen: CHO cells transfected with the B6 allele of Ly49F
other info2 :
Characterization: To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
products references :
1. Coles, M.C., C.W. McMahon, H. Takizawa, and D.H. Raulet. 2000. Eur. J. Immunol. 30:236. 2. Ljunggren, H.G., and K. Karre. 1990. Immunol. Today 11:237. 3. Liu, S., and D.H. Raulet. Personal communication.