product summary
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company name :
MyBioSource
product type :
antibody
product name :
Rat Anti-Mouse CD86/B7-2-PE
catalog :
MBS673454
quantity :
0.1 mg
price :
255 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
GL1
reactivity :
sheep
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
product information
catalog number :
MBS673454
products type :
Secondary Antibody
products full name :
Rat Anti-Mouse CD86/B7-2-PE
products short name :
CD86/B7-2
products name syn :
Rat Anti-Mouse CD86/B7-2 - Phycoerythrin-Labeled; Rat Anti-Mouse CD86/B7-2
other names :
CD86/B7-2 costimulatory molecule; CD86/B7-2 costimulatory molecule; CD86 antigen; B7-1 costimulatory molecule
other gene names :
LOC554318
clonality :
Monoclonal
isotype :
Rat (Louvain) IgG2a kappa
clone :
GL1
host :
Rat
reactivity :
Mouse
sequence length :
325
specificity :
Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4. CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.^1-5 CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation.^1, 5 CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.^1-9 The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.^1, 3, 5
form :
R - phycoerythrin (R - PE) Conjugate
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
app notes :
Research Application(s): In vivo and in vitro blocking assays. Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells
other info1 :
Immunogen: LPS-activated mouse B cells
other info2 :
Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative flow data are included in this product insert.
products references :
1. Hathcock, K., G. Laszlo, C. Pucello, O. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631. 2. Freeman, G.J., F. Borriello, R.J. Hodes, H. Reiser, K.S. Hathcock, G. Laszlo, A.J. McKnight, J. Kim, L. Du, D.B. Lombard, G.S. Gray, L.M. Nadler, and A.H. Sharpe. 1993. Science 262:907. 3. Inaba, K. M. Witmer-Pack, M. Inaba, K.S. Hathcock, H. Sakuta, et al. 1994. J. Exp. Med. 180:1849. 4. Larsen, C.P., S.C. Ritchie, R. Hendrix, P.S. Linsley, K.S. Hathcock, R.J. Hodes, R.P. Lowry, and T.C. Pearson. 1994. J. Immunol. 152:5208. 5. Hathcock, K.S., G. Laszlo, C. Pucillo, P. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631. 6. Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. J. Immunol. 150:5252. 7. Hathcock, K., G. Laszlo, H. Dickler, J. Bradshaw, P. Linsley, and R Hodes. 1993. Science 262:905. 8. Thompson, C.B. 1995. Cell 978:982. 9. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immuol. Today 15:321. 10. Han, S. et. al. 1995. J. Immunol. 155:556.
ncbi gi num :
84000438
ncbi acc num :
NP_001033105.1
ncbi gb acc num :
NM_001038016.1
size1 :
0.1 mg
price1 :
255 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
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