antibody

Rat Anti-Mouse CD80/B7-1-PE

MBS673446

0.1 mg

255 USD

monoclonal

rat

PE

1G10

immunoprecipitation, flow cytometry

Secondary Antibody

Rat Anti-Mouse CD80/B7-1-PE

CD80/B7-1

Rat Anti-Mouse CD80/B7-1 - Phycoerythrin-Labeled; Rat Anti-Mouse CD80/B7-1

CD80/B7-1 transmembrane form; CD80/B7-1 transmembrane form

LOC554317

Monoclonal

Rat IgG2a kappa

1G10

Rat

Mouse

302

Mouse CD80 (B7-1), Mr 45-47 kDa. CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL- 4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. ^1-4 The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex.^5, 6 The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. ^2, 7

R - phycoerythrin (R - PE) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Flow Cytometry; Immunoprecipitation

Research Application(s): In vivo and in vitro blocking of ligand binding. Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.

1. Nabavi, N., G. J. Freeman, A. Gault, D. Godfrey, L. M. Nadler, and L. H. Glimcher. 1992. Nature 360:266. 2. Chen, C., D. A. Faherty, A. Gault, S. E. Connaughton, G. D. Powers, D. I. Godfrey, and N. Nabavi. 1996. J. Immunol. 152:2105. 3. Natesan, M., Z. Razi-Wolf, and H. Reiser. 1996. J. Immunol. 156:2783. 4. Razi-Wolf, Z., G. J. Freeman, F. Galvin, B. Benacerraf, L. Nadler, and H. Reiser. 1992. Proc. Natl. Acad. Sci. U. S. A. 89:4210. 5. Hathcock, K. S., G. Laszlo, C. Pucillo, P. Linsley, and R. J. Hodes. 1994. J. Exp. Med. 180:631. 6. Bluestone, J.A. 1995. Immunity 2:255. 7. Powers, G. D., D. A. Faherty, S. E. Connaughton, D. A. Biondi, D. I. Godfrey, A. Gault, C. Y. Chen, and N. Nabavi. 1994. Cell Immunol. 153:298.

68163383

NP_001020134.1

NM_001024963.1

0.1 mg

255 USD