secondary antibody

Rat Anti-Mouse CD54/ICAM-1-BIOT

MBS673412

0.5 mg

315 USD

Secondary Antibody

Rat Anti-Mouse CD54/ICAM-1-BIOT

CD54 (ICAM-1)

Rat Anti-Mouse CD54/ICAM-1 - Biotin; Rat Anti-Mouse CD54 (ICAM-1)

Monoclonal

Rat IgG2b kappa

YN1/1.7.4

Rat

Mouse

Mouse CD54/ICAM-1, Mr 95 kDa. CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN- gamma, IL-1, TNF- alpha, lipopolysaccharide and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1.

Biotin (BIOT) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and Allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot

Research Application(s): In vitro blocking studies. Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells

Characterization: To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.

1. Takei, F. 1985. J. Immunol. 134:1403. 2. Horley, K.J., C. Carpenito, B. Baker, F. Takei. 1989. EMBO. J. 8:2889. 3. Welder, Clayton A., D.H.S. Lee, F. Takei. 1993. J. Immunol. 150:2203. 4. Qin, Lan, et. al. 1996. J. Immunol. 157:5016.

0.5 mg

315 USD