antibody

Rat Anti-Mouse CD31/PECAM-1-UNLB

MBS673319

0.5 mg

260 USD

monoclonal

rat

nonconjugated

390

immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

Secondary Antibody

Rat Anti-Mouse CD31/PECAM-1-UNLB

CD31/PECAM-1

Rat Anti-Mouse CD31/PECAM-1 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD31/PECAM-1

Monoclonal

Rat (Lewis) IgG2a kappa

390

Rat

Mouse

Mouse CD31/PECAM-1, Mr 130-140 kDa. CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and paltelets.^1-3 CD31 interacts homotypically in cell adhesion assays. MAb 390 inhibits the aggregation of L cells transfected with a variant form of CD31.^1, 3

Purified (UNLB) Antibody

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation

Research Application(s): Blocking of Cell Adhesion. Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells

Immunogen: Mouse leukocyte cell line 32D

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform with characteristics of a standard reference reagent. Representative data are included in this product insert.

1. Abelda, S.M. Personal communication. 2. Buck, C.A., H.S. Baldwin, H. DeLisser, C. Mickanin, H.-M. Shen, G. Kennedy, A. Chen, J.M. Edelman, and S.M. Abelda. 1993. Comptes Rendus de l-Academie des Sciences Serie III-Sciences de la Vie-Life Sciences 316:838. 3. DeLisser, H.M., P.J. newman, and S.M. Abelda. 1993. Curr. Topics Microbiol. Immunol. 184:37.

0.5 mg

260 USD