antibody

Rat Anti-Mouse CD4/L3T4-FITC

MBS673155

0.5 mg

240 USD

monoclonal

rat

FITC

GK1.5

immunohistochemistry, immunoprecipitation, flow cytometry

Secondary Antibody

Rat Anti-Mouse CD4/L3T4-FITC

CD4/L3T4

Rat Anti-Mouse CD4/L3T4 - FITC; Rat Anti-Mouse CD4/L3T4

CD4 antigen; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; OTTMUSP00000027399; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); CD4 antigen; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3

Cd4; Cd4; L3T4; Ly-4

CD4_MOUSE

Monoclonal

Rat (Lewis) IgG2b kappa

GK1.5

Rat

Mouse

457

CD4/L3T4a, Mr 52 kDa. CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested.^1,2 CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. ^3-6 The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. ^1,2 In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4^+ cells.^7,8 The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.^1,8-13

Fluorescein (FITC) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (RPE- CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5 Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Flow Cytometry; Immunohistochemistry; Immunoprecipitation

Research Application(s): In vivo and in vitro depletion of CD4^+ cells. Inhibition of T helper function. Fractionation of CD4^+ cells. Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Immunogen: Cloned mouse CTL line V4

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this package insert.

1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29. 2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933. 3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473. 4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller-Sieburg. 1992. Blood 60:1717. 5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71. 6. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1. 7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. 8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445. 9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118. 10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808. 11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5. 12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131. 13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207. 14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1. 15. Jia, W. and J. F. Kearney. 1996. Personal communication.

24659577

P06332

51,297 Da

Adaptive Immunity Signaling Pathway (366714); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (366720); Cytokines And Inflammatory Response (BioCarta) Pathway (198283); Downstream TCR Signaling Pathway (366719); Generation Of Second Messenger Molecules Pathway (366718); Hematopoietic Cell Lineage Pathway (83275)

Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Subunit structure: Associates with p56-LCK. Interacts with SPG21. Subcellular location: Membrane; Single-pass type I membrane protein. Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.5 mg

240 USD