antibody

Rat Anti-Mouse CD3e-UNLB

MBS673145

0.5 mg

230 USD

monoclonal

rat

nonconjugated

C363.29B (YCD3-1)

immunohistochemistry, immunoprecipitation, flow cytometry

Secondary Antibody

Rat Anti-Mouse CD3e-UNLB

CD3 epsilon

Rat Anti-Mouse CD3e - Purified, Unlabeled Antibody; Rat Anti-Mouse CD3 epsilon

CD3 epsilon; CD3 epsilon

cd3e

Monoclonal

Rat IgG2b kappa

C363.29B (YCD3-1)

Rat

Mouse

172

Mouse CD3 epsilon (Mr 25 kD). CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.^1, 2 CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.^1 In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.^1, 3, 4 The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.^1

Purified (UNLB) Antibody

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Flow Cytometry; Immunohistochemistry; Immunoprecipitation

Research Application(s): Identification and enumeration of CD3^+ cells by flow cytometry. In vitro depletion of CD3^+ cells. In vitro activation of T cells (accessory cells must be provided)

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent by flow cytometry. Representative data are included in this data sheet.

1. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J. Immunol. 142:4169. 2. Dianzani, U., M. Luqman, J. Rojo, J. Yagi, J. L. Baron, A. Woods, C. A. Janeway, Jr., and K. Bottomly. 1990. Eur. J. Immunol. 20:2249. 3. Luqman, M., L. Greenbaum, D. Lu, and K. Bottomly. 1992. Eur. J. Immunol. 22:95. 4. Ernst, D. N., M. V. Hobbs, B. E. Torbett, A. L. Glasebrook, M. A. Rehse, K. Bottomly, K. Hayakawa, R. R. Hardy, and W. O. Weigle. 1990. J. Immunol. 145:1295. 5. Payer, E., A. Elbe, and G. Stingl. 1991. J. Immunol. 146:2536.

83921647

NP_001033071.1

NM_001037982.1

0.5 mg

230 USD