antibody

Mouse Anti-Human CD49d/VLA-4-FITC

MBS670394

100 Tests

240 USD

monoclonal

mouse

FITC

[7.2]

Antibody

Mouse Anti-Human CD49d/VLA-4-FITC

[CD49d/VLA-4]

[VLA-4, Integrin alpha4, VLA-4alpha, ITGA4, IA4]

m

IgG1 kappa

[7.2]

Mouse

Human

Human CD49d; Mr 150kDa

Fluorescein (FITC) Conjugate

The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C

FC-Quality tested 5-6 . ICC-Reported in literature 1-3 . IP- Reported in literature 7 . WB- Reported in literature 4 . Flow Cytometry . For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL. Other applciations vary, you should determine the optimum working dilution for the product that is appropiate for your specific need.

Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.

Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

CD Markers and Other Cell Surface Proteins

CD49d, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin ?4 chain. CD49d associates noncovalently with CD29, the integrin ?1 subunit, to form the ?4?1 very late antigen-4 (VLA-4) heterodimer. Integrin ?4 can also associate with integrin ?7 to form the ?4?7 heterodimer. It is expressed on most leukocytes with the possible exception of neutrophils. VLA-4 mediates leukocyte binding to CD106 (VCAM-1) and is involved in the migration of leukocytes from blood to tissue at sites of inflammation. VLA-4 can also provide a costimulatory signal to T cells for activation and proliferation. The ?4?7 integrin heterodimer is expressed on a small subpopulation of thymocytes, most lymph node T and B lymphocytes, NK cells, and eosinophils. Integrin ?4?7 also binds to fibronectin and VCAM-1

1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD49d Section, Academic Press, New York, p. 262. 2. Lobb, R.R., and M.E. Hemler. 1994. J. Clin. Invest. 94:1722. 3. Alon, R., P.D. Kassner, M.W. Carr, E.B. Finger, M.E. Hemler, and T.A. Springer. 1995. J. Cell. Biol. 128:1243. 4. Berlin, C.,R.F. Bargatze, J.J. Campbell, U.H. von Andrian, M.C. Szabo, S.R. Hasslen, R.D. Nelson, E.L. Berg, S.L. Erlandsen, and E.C. Butcher. 1995. Cell 80:413. 5. Leitinger, B., et al., 2002, J. Cell. Sci. 115:963-72. PMID: 11870215 6. McDowall, A., et al., 2003, J Clin Invest. 111 (1):51-60. PMID: 12511588

100 Tests

240 USD