Rat Anti-Mouse IFN-g-FITC | MyBioSource MBS670352 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse IFN-g-FITC

catalog :

MBS670352

quantity :

0.1 mg

price :

235 USD

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

XMG1.2

reactivity :

mouse

application :

ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS670352

products type :

Antibody

products full name :

Rat Anti-Mouse IFN-g-FITC

products short name :

IFN-g

products name syn :

Rat Anti-Mouse IFN-gamma

other names :

interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; gamma interferon; interferon gamma

other gene names :

Ifng; Ifng; Ifg; IFN-g; IFN-gamma

uniprot entry name :

IFNG_MOUSE

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

XMG1.2

host :

Rat

reactivity :

Mouse

specificity :

Mouse Interferon-gamma (IFN-gamma) This is a neutralizing antibody

form :

Fluorescein (FITC) Conjugate

storage stability :

The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.

tested application :

ELISA

app notes :

ELISA: XMG1.2 monoclonal antibody can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-gamma protein levels. Biotinylated XMG1.2 antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-gamma as the standard.; Western Blot: The purified XMG1.2 antibody is also useful for Western Blot applications. For Western Blot, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, has been found to enable detection of <= 100 ng/lane of recombinant mouse IFN-gamma under reducing conditions.; Immunofluorescence/Flow Cytometry: XMG1.2 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN-gamma-positive cells within mixed cell populations.4-6 Recombinant mouse IFN-gamma or Rat IgG1 should be used as a specificity control. FITC conjugated XMG1.2 antibodies are useful for intracellular staining in cells producing IFN-gamma. Intracellular staining buffers are recommended for this application.; Neutralization: The LE/AF form of XMG1.2 can be used for neutralizing mouse IFN-gamma bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

other info1 :

Immunogen: E. coli-expressed recombinant mouse IFN-gamma. Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

other info2 :

Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

products references :

1. Cherwinski, H.M., J.H. Schumacher, K.D. Brown, and T.R. Mossman. 1987. J. Exp. Med. 166:1229. 2. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5. 3. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201. 4. Vickingson, A., K. Pederson, and D. Muller. 1994. J. Immunol. Meth. 173:219. 5. Ferrick, D.A., M.D. Schrenzel, T. Mulvania, B. Hsieh, W.G. Ferlin, and H. Lepper. 1995. Nature 373:255. 6. Hsieh, B., M. Schrenzel, T. Mulvania, H. Lepper, L. DiMolfetto-Landon, and D. Ferrick. 1996. J. Immunol. 156:232.

ncbi gi num :

33468859

ncbi acc num :

NP_032363.1

ncbi gb acc num :

NM_008337.3

uniprot acc num :

P01580

ncbi mol weight :

17,907 Da

ncbi pathways :

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795)

ncbi summary :

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

uniprot summary :

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Subunit structure: Homodimer. Subcellular location: Secreted. Tissue specificity: Released primarily from activated T lymphocytes. Sequence similarities: Belongs to the type II (or gamma) interferon family.

size1 :

0.1 mg

price1 :

235 USD

more info or order :

MyBioSource product webpage