antibody

Mouse Anti-Human Glycophorin A-UNLB

MBS670232

0.1 mg

165 USD

monoclonal

mouse

nonconjugated

CMRF14

Antibody

Mouse Anti-Human Glycophorin A-UNLB

Glycophorin A

Mouse Anti-Human Glycophorin A

glycophorin-A; Glycophorin-A; glycophorin-A; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Erik; glycophorin A, GPA; MN sialoglycoprotein; glycophorin Sta type C; sialoglycoprotein alpha; Mi.V glycoprotein (24 AA); glycophorin A (MN blood group); recombinant glycophorin A-B Miltenberger-DR; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MNS blood group); MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha

GYPA; GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C); GPA

GLPA_HUMAN

Monoclonal

IgG2b

CMRF14

Mouse

Human

Human glycophorin A. Glycophorin A (GPA) is a 36 kDa sialoglycoprotein that is the most predominant sialoglycoprotein of the erythroid lineage. GPA is the major contributor to the net negative surface charge of the mature red blood cell (RBC) membrane thereby minimizing cell to cell interactions in circulation. The monoclonal antibody CMRF14 binds to glutaraldehyde fixed or neuraminidase treated erythrocytes but not to papain, trypsin or pronase treated erythrocytes.1, 2

Purified (UNLB) Antibody

0.1mg/ml

The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.

Flow Cytometry

Flow Cytometry: . Purified (UNLB) antibody: <= 1 ug/10^6 cells. PE conjugate: <= 10ug/10^6 cells

Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.

Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

1. Chasis, J.A., and N. Mohandas. 1992. Blood. 80:1869. 2. Langlois, R.G., W.L. Bigbee, and R.H. Jensen. 1985. J. Immunol. 134:4009.

298286506

NP_002090.4

NM_002099.6

P02724

16,331 Da

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Malaria Pathway (152665); Malaria Pathway (152657)

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Function: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Ref.21 Ref.23 Ref.26 Ref.29 Ref.30 Ref.32. Subunit structure: Homodimer. Interacts with Streptococcus gordonii hsa protein. Ref.19 Ref.24 Ref.31. Subcellular location: Cell membrane; Single-pass type I membrane protein. Note: Appears to be colocalized with SLC4A1. Ref.25. Post-translational modification: The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-2-6]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-2-6]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-1-2]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-1-2]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). Polymorphism: Along with GYPB, GYPA is responsible for the MNS blood group system. The molecular basis of the GPA M/N bloodgroup antigen is a variation at positions 20 and 24. Ser-20 and Gly-24 correspond to M (shown); 'Leu-20' and 'Glu-24' correspond to N.GYPA polymorphisms are involved in resistance to malaria [. MIM:611162]. Miscellaneous: Involved in several unequal homologous recombinations or gene conversion events, predominantly with GYPB and more rarely with GYPE. The resulting fusion proteins are observed in different phenotypes and encode low incidence bloodgroup antigens. Sequence similarities: Belongs to the glycophorin A family. Sequence caution: The sequence AAA52624.1 differs from that shown. Reason: Erroneous initiation.

0.1 mg

165 USD