catalog number :
MBS670153
products full name :
Mouse Anti-Human MMP-3-HRP
products short name :
MMP-3
products name syn :
Mouse Anti-Human MMP-3 - HRP; Mouse Anti-Human MMP-3
other names :
MMP-3; MMP-3; stromelysin-1; metalloproteinase 3 receptor
other gene names :
MMP3; bmmp-3
uniprot entry name :
Q9N283_BOVIN
specificity :
Human MMP-3; Does not cross react to human MMP-1, MMP-2 or MMP-9. Matrix metalloproteinases (MMPs) are a family of at least 20 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-3, also known as stomelysin-1 and transin, belongs to the stromelysin group of MMP's which also includes MMP-10. MMP-3 has shown direct and indirect substrate specificity to collagens III, IV, V and IX, gelatins, fibronectin, fibrinogen, plasminogen, proteoglycans, elastin, casein, entactin, myelin basic protein, IL-1b, serum amyloid A, TIMP-2, MMP-1, MMP-7, MMP-8, MMP-9, and MMP-13. 1-17
form :
Horseradish Peroxidase (HRP) Conjugate
storage stability :
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. Reagents are stable for the period shown on the label if stored as directed.
tested application :
ELISA; Immunohistochemistry_PES
app notes :
Immunohistochemistry Suggested Dilution: 1:1,000-1:2,000. ELISA Suggested Dilution: 1:1,000-1:4,000
other info1 :
Immunogen: Recombinant MMP-3
other info2 :
Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA to conform with the characteristics of a standard reference reagent.
products references :
1. Nicholson, R., G. Murphy, and R. Breathnach. 1989. Biochemistry 28:5195. 2. Murphy, G., M.I. Cockett, R.V. Ward, and A.J. Docherty. 1991. Biochem. J. 277:277. 3. Okada, Y., H. Konomi, T. Yada, K. Kimata, and H. Nagase. 1989. FEBS Lett. 244:473. 4. Chin, J.R., G. Murphy, and Z. Werb. 1985. J. Biol. Chem. 260:12367. 5. Windsor, L.J., H. Grenett, B. Birkedal-Hansen, M.K. Bodden, J.A. Engler, and H. Birkedal-Hansen. 1993. J. Biol. Chem. 268:17341. 6. Bini, A., Y. Itoh, B.J. Kudryk, and H. Nagase. 1996. Biochemistry 35:13056. 7. Lijnen, H.R., F. Ugwu, A. Bini, and D. Collen. 1998. Biochemistry 37:4699. 8. Sires, U.I., G.L. Griffin, T.J. Broekelmann, R.P. Mecham, G. Murphy, A.E. Ching, H.G. Welhus, and R.M. Senior. 1993. J. Biol. Chem. 268:2069. 9. Chandler, S., R. Coates, A. Gearing, J. Lury, G. Wells, and E. Bone. 1995. Neurosci. Lett. 201:223. 10. Ito, A., A. Mukaiyama, Y. Itoh, H. Magase, I.B. Thogersen, J.J. Enghild, Y. Sasaguri, and Y. Mori. 1996. J. Biol. Chem. 271:14657. 11. Mitchell, T.I., J.J. Jeffrey, R.D. Palmiter, and C.E. Brinckerhoff. 1993. Biochim. Biophys. Acta. 1156:245. 12. Murphy, G., M.I. Cockett, P.E. Stephens, B.J. Smith, and A.J. Docherty. 1987. Biochem. J. 248:265. 13. Miyazaki, K., F. Umenishi, K. Funahashi, N. Koshikawa, U. Yasumitsu, and M. Umeda. 1992. Biochem. Biophys. Res. Commun. 185:852. 14. Imai, K., Y. Yokohama, I. Nakanishi, E. Ohuchi, Y. Fujii, N. Nakai, and Y. Okada. 1995. J. Biol. Chem. 270:6691. 15. Knauper, V., S.M. Wilhelm, P.K. Seperack. Y.A. DeClerck, K.E. Langley, A. Osthues, and H. Tschesche. 1993. Biochem. J. 295:581. 16. Shapiro, S.D., C.J. Fliszar, T.J. Broekelmann, R.P. Mecham, R.M. Senior, and H.G. Welgus. 1995. J. Biol. Chem. 270:6351. 17. Knauper, V., C. Lopez-Otin, B. Smith, G. Knight, and G. Murphy. 1996. J. Biol. Chem. 271:1544.
ncbi mol weight :
16,673 Da
ncbi pathways :
Matrix Metalloproteinases Pathway 198565!!Rheumatoid Arthritis Pathway 200308!!Rheumatoid Arthritis Pathway 200269