Mouse Anti-Human ZAP-70-UNLB | MyBioSource MBS670105 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human ZAP-70-UNLB

catalog :

MBS670105

quantity :

0.1 mg

price :

230 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SBZAP

reactivity :

human, mouse

application :

flow cytometry

product information

catalog number :

MBS670105

products type :

Antibody

products full name :

Mouse Anti-Human ZAP-70-UNLB

products short name :

ZAP-70 (TCR zeta)

products name syn :

Mouse Anti-Human ZAP-70 - Purified, Unlabeled Antibody; Mouse Anti-Human ZAP-70 (TCR zeta)

other names :

ZAP-70; Tyrosine-protein kinase ZAP-70; tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; zeta-chain (TCR) associated protein kinase; 70 kDa zeta-associated protein; Syk-related tyrosine kinase

other gene names :

Zap70; Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364; Srk; Zap-70

uniprot entry name :

ZAP70_MOUSE

clonality :

Monoclonal

isotype :

Mouse IgG1 kappa

clone :

SBZAP

host :

Mouse

reactivity :

Human

specificity :

Human TCR zeta ; Zap-70 (Mr 70 kDa). ZAP 70, also known as TCR zeta associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 amino acid residues 280-309.

form :

Purified (UNLB) Antibody

storage stability :

The purified antibody is supplied as 0.025 mg purified immunoglobulin in 0.25 mL or 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at - 20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL in PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

tested application :

ELISA; Flow Cytometry

app notes :

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

other info1 :

Antigen Description: Human ZAP-70 amino acid residues 280-309

other info2 :

Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA assay to conform to the characteristics of a standard reference reagent.

products references :

1. Wange, R.L., N. Isakov, T.R. Burke., A. Otaka, P.P. Roller, J.D. Watts, R. Aebersold, and L.E. Samelson. 1995. J. Biol. Chem. 270:944. 3. Van Oers, N.S., and A. Weiss. 1995. Semin. Immunol. 7:227.

ncbi gi num :

436480

uniprot acc num :

P43404

ncbi mol weight :

70,058 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway 366714!!B Cell Receptor Signaling Pathway 198285!!Generation Of Second Messenger Molecules Pathway 366718!!Immune System Pathway 366713!!Inflammatory Response Pathway 198280!!Natural Killer Cell Mediated Cytotoxicity Pathway 83276!!Natural Killer Cell Mediated Cytotoxicity Pathway 490!!Primary Immunodeficiency Pathway 83318!!Primary Immunodeficiency Pathway 537!!T Cell Receptor Signaling Pathway 198362

uniprot summary :

Function: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Ref.3. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subunit structure: Interacts with CBL. Interacts with SHB. Interacts with FCRL3. By similarity. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with SLA and SLA2 when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with CBLB and DEF6. Interacts (via SH2 domains) with RHOH. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10. Subcellular location: Cytoplasm. Cell membrane. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. Ref.3 Ref.10. Tissue specificity: Isoform 1 and isoform 2 are expressed in thymus, sleen and lymph nodes. Ref.3. Developmental stage: Isoform 2 is expressed in develoing thymocytes from the CD44+CD25- stage up to mature T cells. Isoform 1 is not expressed in thymocytes at the CD44+CD25- or CD44+CD25+ stages. Ref.3. Domain: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. Ref.10. Post-translational modification: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-318 phosphorylation is essential for full activity. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.

size :

0.1 mg

price :

230 USD