Rat Anti-Mouse IL-6-BIOT | MyBioSource MBS670077 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse IL-6-BIOT

catalog :

MBS670077

quantity :

0.5 mg

price :

295 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP5-32C11

reactivity :

mouse, rat

application :

western blot, ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS670077

products type :

Antibody

products full name :

Rat Anti-Mouse IL-6-BIOT

products short name :

IL-6

products name syn :

Rat Anti-Mouse IL-6 - Biotin; Rat Anti-Mouse IL-6

other names :

interleukin-6; Interleukin-6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); interleukin 6

other gene names :

Il6; Il6; ILg6; Ifnb2; Il-6

uniprot entry name :

IL6_RAT

clonality :

Monoclonal

isotype :

Rat IgG2a kappa

clone :

MP5-32C11

host :

Rat

reactivity :

Mouse

sequence length :

211

specificity :

Mouse Interleukin-6 (IL-6)1-4 This is a non-neutralizing antibody.

form :

Biotin (BIOT) Conjugate

storage stability :

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. Reagents are stable for the period shown on the label if stored as directed.

tested application :

ELISA; Western Blot

app notes :

Research Application(s): ELISA Detection: MP5-32C11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-6 protein levels.1,2,4 Purified MP5-20F3 antibody should be paired with biotinylated as the capture antibody, with purified recombinant mouse IL-6 as the standard. Western Blotting: The purified MP5-32C11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-6 under reducing conditions. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

other info1 :

Immunogen: COS-7-expressed recombinant mouse IL-6

other info2 :

Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

products references :

1. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24. 2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214. 3. Starnes, Jr., A., M. Pearce, A. Tewari, J. Kim, J,-C. Zou, and J. Abrams. 1990. Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-a challenge in mice. J. Immunol. 145:4185-4191. 4. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

ncbi gi num :

7549769

ncbi acc num :

NP_036721.1

ncbi gb acc num :

NM_012589.1

uniprot acc num :

P20607

ncbi mol weight :

24,357 Da

ncbi pathways :

Adipogenesis Pathway 198479!!African Trypanosomiasis Pathway 194377!!African Trypanosomiasis Pathway 194323!!Amoebiasis Pathway 167311!!Amoebiasis Pathway 167191!!Androgen Receptor Signaling Pathway 198529!!Chagas Disease (American Trypanosomiasis) Pathway 147806!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Cytokine-cytokine Receptor Interaction Pathway 83443!!Cytokine-cytokine Receptor Interaction Pathway 460

ncbi summary :

a cytokine involved in development and possibly in neurodegenerative processes [RGD]

uniprot summary :

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-6 superfamily.

size :

0.5 mg

price :

295 USD

more info or order :

MyBioSource product webpage