Rat Anti-Mouse IL-5-UNLB | MyBioSource MBS670070 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse IL-5-UNLB

catalog :

MBS670070

quantity :

0.5 mg

price :

270 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

TRFK5

reactivity :

mouse, cow

application :

western blot, ELISA, immunocytochemistry, flow cytometry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS670070

products type :

Antibody

products full name :

Rat Anti-Mouse IL-5-UNLB

products short name :

IL-5

products name syn :

Rat Anti-Mouse IL-5 - Purified, Unlabeled Antibody; Rat Anti-Mouse IL-5

other names :

interleukin-5; Interleukin-5; interleukin-5; T-cell replacing factor; eosinophil differentiation factor; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; T-cell replacing factor

other gene names :

IL5; IL5; TRF; IL-5

uniprot entry name :

IL5_BOVIN

clonality :

Monoclonal

isotype :

Rat IgG1 kappa

clone :

TRFK5

host :

Rat

reactivity :

Mouse

sequence length :

134

specificity :

Mouse and Human Interleukin-5 (IL-5)1-4 (This is a neutralizing antibody.)

form :

Purified (UNLB) Antibody

storage stability :

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

tested application :

ELISA; Flow Cytometry; Immunofluorescence; Western Blot

app notes :

Research Application(s): ELISA Capture: TRFK5 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified TRFK5 antibody should be paired with biotinylated TRFK4 antibody as the detection antibody, with purified recombinant mouse IL-5 as the standard. Immunofluorescence/Flow Cytometry: TRFK5 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blotting: The purified TRFK5 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-5 under reducing conditions. Neutralization: The LE/AF form of TRFK5 can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

other info1 :

Immunogen: Semi-purified mouse T cell clone supernatant.

other info2 :

Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

products references :

1. Schumacher, J.H., A. O'garra. B. Schrader, A. Van Kimmenade, M.W. Bond, T.R. Mosmann, and R.L. Coffman. 1988. J. Immunol. 141:1576. 2. Swain, S., A. Welberg, M. English, and G. Houston. 1990. J. Immunol. 145:3796. 3. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5. 4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201. 5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20. 6. Mo, X.Y., S. R. Sarawar, and P. Doherty. 1995. J. Virol. 69:1288. 7. Sarawar, S. M. Sangster, R. Coffman, and P. Doherty. 1994. J. Immunol. 153:1246. 8. Assenmacher, M., J. Schmitz, and A. Radbruch. 1994. Eur. J. Immunol. 24:1097.

ncbi gi num :

27806873

ncbi acc num :

NP_776347.1

ncbi gb acc num :

NM_173922.1

uniprot acc num :

P52173

ncbi mol weight :

15,229 Da

ncbi pathways :

Allograft Rejection Pathway 84358!!Allograft Rejection Pathway 535!!Asthma Pathway 84355!!Asthma Pathway 532!!Autoimmune Thyroid Disease Pathway 84356!!Autoimmune Thyroid Disease Pathway 533!!Cytokine Signaling In Immune System Pathway 362320!!Cytokine-cytokine Receptor Interaction Pathway 84290!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response (BioCarta) Pathway 198574

uniprot summary :

Function: Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. Subunit structure: Homodimer; disulfide-linked. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-5 family.

size :

0.5 mg

price :

270 USD

more info or order :

MyBioSource product webpage