Rat Anti-Mouse IL-4-UNLB | MyBioSource MBS670066 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse IL-4-UNLB

catalog :

MBS670066

quantity :

0.5 mg

price :

270 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

BVD4-1D11

reactivity :

pig

application :

western blot, ELISA, immunocytochemistry, flow cytometry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS670066

products type :

Antibody

products full name :

Rat Anti-Mouse IL-4-UNLB

products short name :

IL-4

products name syn :

Rat Anti-Mouse IL-4 - Purified, Unlabeled Antibody; Rat Anti-Mouse IL-4

other names :

interleukin-4; Interleukin-4; interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1

other gene names :

IL4; IL4

uniprot entry name :

IL4_PIG

clonality :

Monoclonal

isotype :

Rat IgG2b kappa

clone :

BVD4-1D11

host :

Rat

reactivity :

Mouse

sequence length :

133

specificity :

Mouse Interleukin-4 (IL-4)1, 2 (This is a neutralizing antibody.)

form :

Purified (UNLB) Antibody

storage stability :

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Reagents are stable for the period shown on the label if stored as directed.

tested application :

ELISA; Flow Cytometry; Immunofluorescence; Western Blot

app notes :

Research Application(s): ELISA Capture: BVD4-1D11 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified BVD4-1D11 antibody should be paired with biotinylated BVD6-24G2 antibody as the detection antibody, with purified recombinant mouse IL-4 as the standard. Immunofluorescence/Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blotting: The purified BVD4-1D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-4 under reducing conditions. Neutralization: The LE/AF form of BVD4-1D11 can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

other info1 :

Immunogen: E. coli-expressed recombinant mouse IL-4

other info2 :

Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

products references :

1. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24. 2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214. 3. Finkelman, F., K. Madden, S. Morris, J. Holmes, N. Bolani,, I. Jkatona, and C. Maliszewski. 1993. Anti-cytokine antibodies as carrier proteins. J. Immunol. 151:1235-1244. 4. Litton, M., B. Sander, E. Murphy, A. O'Garra, and J. Abrams. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J. Immunol. Meth. 175:47-58.

ncbi gi num :

55742622

ncbi acc num :

NP_999288.1

ncbi gb acc num :

NM_214123.1

uniprot acc num :

Q04745

ncbi mol weight :

15,023 Da

ncbi pathways :

Allograft Rejection Pathway 84530!!Allograft Rejection Pathway 535!!Asthma Pathway 84527!!Asthma Pathway 532!!Autoimmune Thyroid Disease Pathway 84528!!Autoimmune Thyroid Disease Pathway 533!!Cytokine-cytokine Receptor Interaction Pathway 84463!!Cytokine-cytokine Receptor Interaction Pathway 460!!Fc Epsilon RI Signaling Pathway 84494!!Fc Epsilon RI Signaling Pathway 493

uniprot summary :

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-4/IL-13 family.

size :

0.5 mg

price :

270 USD

more info or order :

MyBioSource product webpage