Rat Anti-Human IL-7-UNLB | MyBioSource MBS670048 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Human IL-7-UNLB

catalog :

MBS670048

quantity :

0.5 mg

price :

315 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

BVD10-40F6

reactivity :

human

application :

western blot, ELISA, immunoprecipitation

product information

catalog number :

MBS670048

products type :

Antibody

products full name :

Rat Anti-Human IL-7-UNLB

products short name :

IL-7

products name syn :

Rat Anti-Human IL-7 - Purified, Unlabeled Antibody; Rat Anti-Human IL-7

other names :

interleukin-7 isoform 1; Interleukin-7; interleukin-7; OTTHUMP00000227044; OTTHUMP00000227046; interleukin 7

other gene names :

IL7; IL7; IL-7

uniprot entry name :

IL7_HUMAN

clonality :

Monoclonal

isotype :

Rat IgG1 kappa

clone :

BVD10-40F6

host :

Rat

reactivity :

Human

specificity :

Human Interleukin-7 (IL-7)1, 2 (This is a neutralizing antibody)

form :

Purified (UNLB) Antibody

storage stability :

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

tested application :

ELISA; Immunoprecipitation; Western Blot

app notes :

Research Application(s): ELISA Capture: BVD10-40F6 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying human IL-7 protein levels.1, 2 Purified BVD10-40F6 antibody should be paired with biotinylated BVD10-11C10 antibody as the detection antibody, with purified recombinant human IL-7 as the 3standard. Western Blotting: The purified BVD10-40F6 antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with ALPH-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-7 under reducing conditions. Neutralization: The LE/AF form of BVD10-40F6 can be used for neutralizing human IL-7 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

other info1 :

Immunogen: E. coli-expressed recombinant human IL-7

other info2 :

Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

products references :

1. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5. 2. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

ncbi gi num :

4504677

ncbi acc num :

NP_000871.1

ncbi gb acc num :

NM_000880.3

uniprot acc num :

P13232

ncbi mol weight :

20,187 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488

ncbi summary :

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.

uniprot summary :

Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-7/IL-9 family.

size :

0.5 mg

price :

315 USD