Mouse Anti-Human IFN-g-BIOT | MyBioSource MBS670031 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human IFN-g-BIOT

catalog :

MBS670031

quantity :

0.5 mg

price :

295 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B27

reactivity :

human

application :

ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS670031

products type :

Antibody

products full name :

Mouse Anti-Human IFN-g-BIOT

products short name :

IFN- gamma

products name syn :

nterferon-?, gIFN, IFNG, immune interferon, IIF, type II interferon, T interferon, T cell interferon, mitogen induced interferon, pH2-labile interferon, macrophage-activating factor, MAF

other names :

interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; OTTHUMP00000240111; interferon, gamma; Immune interferon

other gene names :

IFNG; IFNG; IFG; IFI

uniprot entry name :

IFNG_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgG1 kappa

clone :

B27

host :

Mouse

reactivity :

Human

sequence length :

166

specificity :

Human Interferon- gamma (IFN- gamma)1-2 This is a neutralizing antibody. B27 monoclonal antibody has been used for epitope mapping of human IFN- gamma 1. The B27 antibody does not bind to denatured IFN- gamma 1.

form :

Biotin (BIOT) Conjugate

storage stability :

The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.

tested application :

ELISA

app notes :

Research Application(s): ELISA Detection: B27 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IFN- gamma protein levels.1,2 Biotinylated B27 antibody should be paired with purified A35 antibody as the capture antibody, with purified recombinant human IFN- gamma as the standard. Immunofluorescence/Flow Cytometry: B27 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN- gamma -positive cells within mixed cell populations. Recombinant human IFN- gamma, unlabeled B27, or Rat IgG1 should be used as a specificity control. Immunoprecipitation: The B27 antibody has been reported to be useful for immunoprecipitation.1. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. ELISA: BIOT conjugate 1:2,000-1:8,000

other info1 :

Immunogen: E. coli-expressed recombinant human IFN- gamma

other info2 :

Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

products references :

1. Favre, C., J. Wijdenes, H. Cabrillat, O. Djossou, J. Banchereau, and J.E. de Vries. 1989. Epitope mapping of recombinant human gamma interferon using monoclonal antibodies. Molec. Immunol. 26:17-25. 2. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.

ncbi gi num :

56786138

ncbi acc num :

NP_000610.2

ncbi gb acc num :

NM_000619.2

uniprot acc num :

P01579

ncbi mol weight :

19,348 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

ncbi summary :

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.

uniprot summary :

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Subunit structure: Homodimer. Subcellular location: Secreted. Tissue specificity: Released primarily from activated T lymphocytes. Post-translational modification: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Involvement in disease: In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [. MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. Pharmaceutical use: Available under the name Actimmune (Genentech). Used for reducing the frequency and severity of serious infections associated with chronic granulomatous disease (CGD). Sequence similarities: Belongs to the type II (or gamma) interferon family.

size1 :

0.5 mg

price1 :

295 USD

more info or order :

MyBioSource product webpage