This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
Rat Anti-Human IL-10-UNLB
catalog :
MBS670020
quantity :
0.5 mg
price :
270 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
JES3-9D7
reactivity :
Sheeppox virus
application :
western blot, ELISA, immunocytochemistry, flow cytometry
product information
catalog number :
MBS670020
products type :
Antibody
products full name :
Rat Anti-Human IL-10-UNLB
products short name :
IL-10
products name syn :
Rat Anti-Human IL-10 - Purified, Unlabeled Antibody; Rat Anti-Human/Viral IL-10
other names :
IL-10; IL-10
products gene name :
IL-10
other gene names :
SPPV_03
clonality :
Monoclonal
isotype :
Rat IgG1 kappa
clone :
JES3-9D7
host :
Rat
reactivity :
Human
sequence length :
168
specificity :
Human and Viral Interleukin-10 (IL-10)1-5 (This is a neutralizing antibody)
form :
Purified (UNLB) Antibody
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
ELISA; Flow Cytometry; Immunofluorescence; Western Blot
app notes :
Research Application(s): ELISA Capture: Purified JES3-9D7 monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified JES3-9D7 antibody should be paired with biotinylated JES3-12G8 antibody as the detection antibody, with purified recombinant human IL-10 as the standard. Immunofluorescence/Flow Cytometry: The JES3-9D7 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control. Western Blotting: The purified JES3-9D7 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-10 under reducing conditions. Neutralization: The LE/AF form of JES3-9D7 can be used for neutralizing human/viral IL-10 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
other info1 :
Immunogen: COS-expressed human IL-10
other info2 :
Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
products references :
1. Abrams, J.S., M.-G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5. 2. Gotlieb, W.H., J.S. Abrams, J.M. Watson, T. Velu, J.S. Berek, and O. Martinez-Maza. 1992. Cytokine 4:385. 3. Yssel, H., R. de Waal Malefyt, M-G. Roncarolo, J. Abrams, R. Lahesmaa, H. Spits, and J. de Vries. 1992. J. Immunol. 149:2378. 4. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20. 5. Burdin, N., C. Peronne, J. Banchereau, and F. Rousset. 1993. J. Exp. Med. 177:295.
ncbi gi num :
21492460
ncbi acc num :
NP_659579.1
ncbi gb acc num :
NC_004002.1
size :
0.5 mg
price :
270 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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