antibody

Mouse Anti-Mouse Bax-UNLB

MBS670006

0.1 mg

250 USD

monoclonal

mouse

nonconjugated

5B7

mouse, rat

western blot, ELISA, immunoprecipitation

Antibody

Mouse Anti-Mouse Bax-UNLB

Bax

Mouse Anti-Mouse Bax - Purified, Unlabeled Antibody; Mouse Anti-Mouse Bax

bax; Apoptosis regulator BAX; apoptosis regulator BAX; Bcl2-associated X protein

Bax; Bax; Bax

BAX_RAT

Monoclonal

Mouse IgG1

5B7

Mouse

Mouse

133

Mouse Bax (Mr 21 kDa). Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program.4-13 Mab 5B7 is specific for mouse Bax.1

Purified (UNLB) Antibody

The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The products should be stored at 2-8 degree C. Each product is stable for the period shown on the label if stored as directed.

ELISA; Immunoprecipitation; Western Blot

Western Blot Suggested Dilution: <= 1 ug/ml

Immunogen: KLH-conjugated peptide corresponding to amino acids 3-16 near the N-terminus of murine Bax 1

Characterization: To insure lot-to-lot consistency, each batch of product is tested by immunoassay to conform to the characteristics of a standard reference reagent.

1. Hsu, Y.T. and R.J. Youle. 1997. J. Biol. Chem. 272:13829. 2. Cohen, J.J. 1991. Adv. Immunol. 50:55. 3. Cohen, J.J., and R.C. Duke. 1992. Annu. Rev. Immunol. 10:2676. 4. Nunez, G., and M.F. Clarke. 1994. Trends Cell Biol. 4:399. 5. Cory, S. 1995. Annu. Rev. Immunol. 13:513. 6. Oltvai., Z.N., C.L. Millman, and S.J. Korsmeyer. 1993. Cell 74:609. 7. Farrow, S.N., et al. 1995. Nature 374:731. 8. Chittenden, T., et al. 1995. Nature 374:733. 9. Kiefer, M.C., et al. 1995. Nature 374:736. 10. Shimizu, S., et al. 1995. Nature 374:811. 11. Yin, X.M., Z.N. Oltvai, and S.J. Korsmeyer. 1994. Nature 369:321. 12. Cheng, E.H.-Y., et al. 1996. Nature 379:554. 13. Gottschalk, A.R., L.H. Boise, Z.N. Oltvai, M.A. Accavitti, S.J. Korsmeyer, J. Quintans, and C.B. Thompson. 1996. Cell Death and Differentiation 3:113.

975870

Q63690

21,351 Da

ATM Pathway 198524!!Activation, Translocation And Oligomerization Of BAX Pathway 368636!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83491!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 219785!!Apoptosis Pathway 83452!!Apoptosis Pathway 470!!Apoptosis Pathway 368621!!B Cell Receptor Signaling Pathway 198486!!Colorectal Cancer Pathway 83494

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]

Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Subunit structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Subcellular location: Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein. By similarity. Cytoplasm. By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. By similarity.Isoform Beta: Cytoplasm. By similarity. Isoform Gamma: Cytoplasm. Tissue specificity: Expressed in a wide variety of tissues, with highest levels in the testis and ovary. Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. Sequence similarities: Belongs to the Bcl-2 family.

0.1 mg

250 USD