Monoclonal Antibody to FSH Receptor (Clone: ABM5D29) | MyBioSource MBS669032 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to FSH Receptor (Clone: ABM5D29)

catalog :

MBS669032

quantity :

0.1 mg

price :

290 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[ABM5D29]

reactivity :

human

application :

western blot, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Fig-1: Western blot analysis of FSH Receptor. Anti-FSH Receptor antibody (Clone: ABM5D29) was used at 2 ug/ml on Hela lysate.

image 2 :

Fig-2: Intracellular flow analysis of FSH receptor antibody on A431 cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).

image 3 :

Fig-3: Intracellular flow analysis of FSH receptor antibody on HeLa cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).

product information

catalog number :

MBS669032

products type :

Antibody

products full name :

Monoclonal Antibody to FSH Receptor (Clone: ABM5D29)

products short name :

[FSH Receptor]

products name syn :

[Follicle-stimulating hormone receptor; FSH-R; Follitropin receptor]

other names :

[follicle-stimulating hormone receptor isoform 1; Follicle-stimulating hormone receptor; follicle-stimulating hormone receptor; follicle stimulating hormone receptor; Follitropin receptor]

products gene name :

[FSHR]

other gene names :

[FSHR; FSHR; LGR1; ODG1; FSHR1; FSHRO; LGR1; FSH-R]

clonality :

Monoclonal

isotype :

IgG1, Kappa

clone :

[ABM5D29]

host :

Mouse

reactivity :

Human

sequence length :

695

purity :

Purified. Protein G Chromatography

form :

25 ug in 50 uL/100 ug in 200 uL PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

storage stability :

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

tested application :

Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

WB: 2-4 ug/ml. FC/FACS: 0.5-1 ug/10^6

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Antigen: FSH Receptor. Immunogen Information: A partial length recombinant protein (a.a 03-200) of FSH Receptor was used as the immunogen for this antibody. Conjugation: Unconjugated

products categories :

Cancer Marker

products description :

FSH receptor is a member of the G protein coupled receptor family consisting of 9 introns and 10 exons and a promoter region and is located on chromosome 2p21. FSH receptor was shown to be expressed selectively on the luminal surface of tumor blood vessels. A general characteristic of the blood vessels that express the endothelial FSH receptor is that they are located at the periphery of the tumors. Many studies showed that FSH receptor polymorphisms at positions 307 and 680 may be even more relevant for clinical practice for the reasons of regulating the ovarian reaction to hormone, controlling ovarian hyperstimulation, changing the menstrual cycle, and causing POF (Premature Ovarian Failure) and PCOS (Polycystic Ovary Syndrome).

ncbi gi num :

31657138

ncbi acc num :

NP_000136.2

ncbi gb acc num :

NM_000145.3

uniprot acc num :

P23945

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); FSH Signaling Pathway (672455); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); GPCRs, Other Pathway (198765); Hormone Ligand-binding Receptors Pathway (106369); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

uniprot summary :

Receptor for follicle-stimulating hormone or follitropin (PubMed:11847099, PubMed:24058690). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099). Induces cAMP production through the activation of PI3K-AKT and SRC-ERK1/2 signaling pathways (PubMed:24058690).

size1 :

0.1 mg

price1 :

290 USD

more info or order :

MyBioSource product webpage