Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16) | MyBioSource MBS669029 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16)

catalog :

MBS669029

quantity :

0.1 mg

price :

290 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[ABM5A16]

reactivity :

human

application :

western blot, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Figure-1: Western blot analysis of Spectrin beta 3. Anti-Spectrin beta 3 antibody (Clone: ABM5A16) was used at 0.1 ug/ml on h Kidney and h Brain lysates.

image 2 :

Figure-2: Intracellular flow analysis of human Spectrin beta 3 in A431 cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).

image 3 :

Figure-3: Intracellular flow analysis of human Spectrin beta 3 in HeLa cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).

product information

catalog number :

MBS669029

products type :

Antibody

products full name :

Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16)

products short name :

[Spectrin beta 3]

products name syn :

[Spectrin beta chain; non-erythrocytic 2; Beta-III spectrin; Spinocerebellar ataxia 5 protein]

other names :

[spectrin beta chain, non-erythrocytic 2; Spectrin beta chain, non-erythrocytic 2; spectrin beta chain, non-erythrocytic 2; spectrin beta, non-erythrocytic 2; Beta-III spectrin; Spinocerebellar ataxia 5 protein]

products gene name :

[SPTBN2]

other gene names :

[SPTBN2; SPTBN2; SCA5; SCAR14; GTRAP41; KIAA0302; SCA5]

clonality :

Monoclonal

isotype :

IgG2a, Kappa

clone :

[ABM5A16]

host :

Mouse

reactivity :

Human

sequence length :

2390

purity :

Purified. Protein G Chromatography

form :

Purified. 25 ug in 50 uL/100 ug in 200 uL in PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.

storage stability :

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

tested application :

Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

WB: 0.1-0.5 ug/ml. FACS: 2-4 ug/10 6

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Immunogen : A partial length recombinant protein (a.a 1280-1450) of Spectrin beta 3 was used as the immunogen for this antibody.

products categories :

Cancer Marker

products description :

Beta-III spectrin is a protein encoded by the gene SPTBN2, spans 2390 amino acids and consists of an amino-terminal actin binding domain (ABD), a central region containing seventeen spectrin repeat domains, and a carboxy-terminal pleckstrin homology domain. It is critical for the correct development and maintenance of Purkinje cell dendritic structure. Beta-III spectrin is expressed predominantly in the brain and is enriched in cerebellar Purkinje cells. In addition, Beta-III spectrin participates in endomembrane trafficking through its interaction with the actin related protein, ARP1. The functional unit of Beta-III spectrin is considered to be a tetrameric complex composed of two Beta-spectrin subunits and two Alpha-II-spectrin subunits. Mutations in the gene encoding Beta-III spectrin give rise to spinocerebellar ataxia type 5, a neurodegenerative disease characterized by progressive thinning of the molecular layer, loss of Purkinje cells and increasing motor deficits.

ncbi gi num :

5902122

ncbi acc num :

NP_008877.1

ncbi gb acc num :

NM_006946.3

uniprot acc num :

O15020

ncbi mol weight :

268,309 Da

ncbi pathways :

Axon Guidance Pathway (575211); Axon Guidance Pathway (105688); Developmental Biology Pathway (575210); Developmental Biology Pathway (477129); Interaction Between L1 And Ankyrins Pathway (161005); L1CAM Interactions Pathway (161003); NCAM Signaling For Neurite Out-growth Pathway (105689); NCAM Signaling For Neurite Out-growth Pathway (575220)

ncbi summary :

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]

uniprot summary :

Probably plays an important role in neuronal membrane skeleton.

size1 :

0.1 mg

price1 :

290 USD

more info or order :

MyBioSource product webpage