Monoclonal Antibody to BST 2 (Clone: ABM52D8) | MyBioSource MBS668285 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to BST 2 (Clone: ABM52D8)

catalog :

MBS668285

quantity :

0.1 mg

price :

290 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[ABM52D8]

reactivity :

human

application :

western blot, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Fig-1: Cell surface flow analysis of BST2 in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).

image 2 :

Fig-2: Cell surface flow analysis of BST2 on Molt4 cells using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).

image 3 :

Fig-3: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 ug/ml on Ramos lysate.

product information

catalog number :

MBS668285

products type :

Antibody

products full name :

Monoclonal Antibody to BST 2 (Clone: ABM52D8)

products short name :

[BST 2]

products name syn :

[Bone marrow stromal antigen 2; HM1.24 antigen; Tetherin; CD317]

other names :

[Bone marrow stromal antigen 2; Bone marrow stromal antigen 2; bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; HM1.24 antigen; Tetherin; CD_antigen: CD317]

products gene name :

[BST2]

other gene names :

[BST2; BST2; CD317; TETHERIN; BST-2]

clonality :

Monoclonal

isotype :

IgG1 Kappa

clone :

[ABM52D8]

host :

Mouse

reactivity :

Rat, Mouse, Human

sequence length :

180

purity :

Purified. Protein G Chromatography

form :

25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

storage stability :

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

tested application :

Flow Cytometry (FC/FACS), Western Blot (WB)

app notes :

WB: 2-4 ug/ml. Flow Cytometry: 0.5-1 ug/10^6

image1 heading :

Flow Cytometry (FC/FACS)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Fig-4: Western blot analysis of BST 2. Anti-BST 2 antibody (Clone: ABM52D8) was used at 2 µg/ml on (1) m Lung, (2) r Lung, (3) m Kidney and (4) r Kidney lysates.

other info1 :

Immunogen Information: Full length recombinant protein of BST 2 was used as the immunogen for this antibody.

products categories :

Antibodies; Immunology

products description :

BST2 (Bone Marrow Stromal Antigen 2), a type II transmembrane protein also known as HM1.24/CD317, has been identified to be overexpressed in a variety of cell lines from different cancer types, including multiple myeloma, breast, lung, and kidney cancers. BST2 was discovered as a marker of differentiated B cells and was later rediscovered as a potent antiviral restriction factor with the ability to tether enveloped viruses to the cell membrane of infected cells via its GPI anchor as well as to potently inhibit virus replication in cultured cells and in vivo. It also acts as an innate immune sensor of HIV-1 that elicits an inflammatory response upon exposure. BST2 mediate host immune response by activating NF-KB through interaction with TAK1 (Transforming Growth Factor Beta-Activated Kinase 1) and TNF receptor associated factors (TRAFs) 2 and 6. In addition, BST2 induces ADCC (antibody-dependent cell cytotoxicity) against the envelope protein of HIV. BST2 was expressed constitutively on the cell surface of both CD3- and CD3+ thymocytes ex vivo.

ncbi gi num :

1705508

ncbi acc num :

Q10589.1

uniprot acc num :

Q10589

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104)

ncbi summary :

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq, Jul 2008]

uniprot summary :

BST2: IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason- Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma- associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, and paramyxoviridae: nipah virus. Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection. Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Belongs to the tetherin family. Protein type: Cell development/differentiation; Membrane protein, GPI anchor; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13.11. Cellular Component: apical plasma membrane; cell surface; cytoplasm; Golgi apparatus; integral to plasma membrane; membrane; multivesicular body; plasma membrane. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protein homodimerization activity; signal transducer activity. Biological Process: cell proliferation; cell-cell signaling; defense response to virus; humoral immune response; innate immune response; multicellular organismal development; negative regulation of cell growth; negative regulation of cell migration; negative regulation of plasmacytoid dendritic cell cytokine production; negative regulation of viral genome replication; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of actin cytoskeleton organization and biogenesis; response to virus

size1 :

0.1 mg

price1 :

290 USD

more info or order :

MyBioSource product webpage