antibody

Monoclonal Antibody to RIG-I (Clone: ABM4H29)

MBS668173

0.1 mg

270 USD

monoclonal

mouse

nonconjugated

[ABM4H29]

western blot, immunohistochemistry, flow cytometry, FACS

Antibody

Monoclonal Antibody to RIG-I (Clone: ABM4H29)

[RIG I /DDx58]

[probable ATP-dependent RNA helicase DDX58; Probable ATP-dependent RNA helicase DDX58; probable ATP-dependent RNA helicase DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; RIG-I-like receptor 1; RLR-1; Retinoic acid-inducible gene 1 protein; RIG-1; Retinoic acid-inducible gene I protein; RIG-I]

[RIG I/DDx58]

[DDX58; DDX58; RIGI; RIG-I; RLR-1; SGMRT2; RLR-1; RIG-1; RIG-I]

DDX58_HUMAN

Monoclonal

Mouse IgG1, Kappa

[ABM4H29]

Mouse

Human

925

Protein G chromatography

Purified; 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Immunohistochemistry (IHC), Flow Cytometry(FACS), Western Blot(WB)

Western blot analysis: 2-4ug/ml. Immunohistochemical analysis: 15ug/ml. Flow Cytometric analysis: 0.5-1ug/10^6 cells

Western Blot (WB)

Immunohistochemical

Immunohistochemical

Flow Cytometry(FC)

Figure-4: Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (10-4121 Abeomics). Goat anti-mouse PE conjugate was used as secondary.

Immunogen: A partial length recombinant human RIG-1 protein (amino acids 1-220) was used as the immunogen for this antibody.

Immunology

RIG-I (retinoic-acid-inducible gene I), also known as DDX58 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 58) is a 925-residue cytoplasmic viral RNA receptor, critically involved in the activation of the innate immune response to RNA virus infection. It is a member of the RIG-I-like receptor (RLR) family and is an essential intracellular sensor for several 5?-triphosphorylated RNA viruses. RIG-I elicits its antiviral interferon (IFN) responses by recognizing viral double-stranded RNAs (dsRNAs). Structurally it comprises a helicase domain, a C-terminal domain, and N-terminal CARDs (caspase activation recruitment domains) involved in activating MAVS (mitochondrial antiviral signaling protein). Upon binding of 5?-triphosphorylated RNA, RIG-I undergoes conformational changes and post-translational modifications that allow multimerization and interaction with the mitochondrial antiviral signaling protein (MAVS).

27881482

NP_055129.2

NM_014314.3

O95786

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis B Pathway (694606); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873)

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]

DDX58: an innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5 - triphosphorylated ssRNA and dsRNA and short dsRNA ( 1 kb in length). In addition to the 5 -triphosphate moiety, blunt-end base pairing at the 5 -end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3 overhang at the 5 triphosphate end decreases and any 5 overhang at the 5 triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein MAVS which activates the IKK- related kinases TBK1 and IKBKE which phosphorylate interferon regulatory factors IRF3 and IRF7, in turn activating transcription of antiviral immunological genes, including IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory synctial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration. Maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58, IKBKE, TBK1 and STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58. Interacts with SRC. Interacts with protein Z of Guanarito virus, Machupo virus, Junin arenavirus and Sabia virus. This interaction disrupts its interaction with MAVS, impeding downstream IRF3 and NF-kappa-B activation and resulting in decreased IFN-beta induction. Interacts (via CARD domain) with Human respiratory syncytial virus A non-structural protein 2 (NS2) and this interaction disrupts its interaction with MAVS, impeding downstream IRF3 activation. Present in vascular smooth cells. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.6.4.13; Hydrolase. Chromosomal Location of Human Ortholog: 9p12. Cellular Component: tight junction; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: identical protein binding; protein binding; single-stranded RNA binding; zinc ion binding; double-stranded RNA binding; double-stranded DNA binding; helicase activity; ATP binding. Biological Process: viral reproduction; metabolic process; response to virus; regulation of cell migration; response to exogenous dsRNA; detection of virus; positive regulation of transcription factor import into nucleus; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of defense response to virus by host; negative regulation of interferon type I production; positive regulation of interferon-alpha production. Disease: Singleton-merten Syndrome 2

0.1 mg

270 USD