CD133/Prominin-1 Polyclonal Antibody | MyBioSource MBS668079 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD133/Prominin-1 Polyclonal Antibody

catalog :

MBS668079

quantity :

0.1 mg

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS668079

products type :

Antibody

products full name :

CD133/Prominin-1 Polyclonal Antibody

products short name :

[CD133/Prominin-1]

products name syn :

[PROM1,PROML1,MSTP061]

other names :

[prominin-1 isoform 2; Prominin-1; prominin-1; antigen AC133; hProminin; hematopoietic stem cell antigen; prominin-like protein 1; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133]

products gene name :

[CD133]

other gene names :

[PROM1; PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; PROML1]

uniprot entry name :

PROM1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

856

purity :

Protein A Chromatography

form :

Purified. 25 ug in 50uL /100 ug in 200 uL PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

storage stability :

Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

tested application :

Western Blot (WB)

app notes :

Western Blot: 1-2 µg/ml

image1 heading :

Western Blot (WB)

other info1 :

Antigen: CD133/Prominin-1

other info2 :

Immunogen: A partial recombinant CD133 protein (amino acids 230-430) was used as the immunogen for this antibody.

products categories :

Development

products description :

CD133/Prominin-1 is a pentaspan transmembrane protein that has been frequently used as a biomarker for cancer stem cells,although its biological function is unclear. CD133 is not restricted to somatic stem cells and cancer stem cells. It is expressed indifferentiated epithelial cells in various organs. Due to the highly restricted expression of CD133 family molecules on plasma membrane protrusions of epithelial and other cell types, in association with membrane cholesterol, a role in the organization of plasma membrane topology has also recently been assigned to this family

ncbi gi num :

224994189

ncbi acc num :

NP_001139319.1

ncbi gb acc num :

NM_001145847.1

uniprot acc num :

O43490

ncbi pathways :

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: extracellular space; stereocilium; cell surface; photoreceptor outer segment; microvillus membrane; integral to plasma membrane; endoplasmic reticulum; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; vesicle; brush border. Molecular Function: actinin binding; protein binding; cadherin binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12

size1 :

0.1 mg

price1 :

270 USD

more info or order :

MyBioSource product webpage