antibody

Caspase-3 (Pro and Active) Monoclonal Antibody

MBS668032

0.1 mg

290 USD

monoclonal

mouse

nonconjugated

[ABM1C12]

human, mouse, rat

Antibody

Caspase-3 (Pro and Active) Monoclonal Antibody

[Caspase-3 (Pro and Active)]

[Casp3,Cpp32]

[caspase-3; Caspase-3; caspase-3; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; cysteine protease CPP32; protein Yama; caspase 3; Apopain; Cysteine protease CPP32; CPP-32]

[Casp3; Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik; Cpp32; CASP-3; CPP-32; SCA-1]

CASP3_MOUSE

Monoclonal

Mouse IgG1, Kappa

[ABM1C12]

Mouse

Human (Predicted Reactivity: Mouse, Rat

277

Protein G Chromatography

Purified. 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western blot analysis: 2-4 ug/ml. Immunohistochemical analysis: 5-10 Ug/ml. FACS analysis: 0.5-1 ug/10^6 cells

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) #2 (IHC)

Immunohistochemistry (IHC) #3 (IHC)

Immunohistochemical analysis of Caspase-3 in normal human spleen tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.

Immunohistochemistry (IHC) #4 (IHC)

Immunohistochemical analysis of Caspase-3 in squamous cell carcinoma of lungs using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.

Antigen: Caspase-3 (Pro and Active)

Immunogen: Full length recombinant Caspase-3 protein was used as the immunogen for this antibody

Apoptosis

Caspases are a member of the cysteine-aspartic acid protease family. Caspase-3 (31 kDa) is an executionary caspase which directly cleaves and activates poly(ADP-ribose) polymerase (PARP), sterol regulatory element binding proteins (SREBPs) or it can also interact with other caspases like caspase-6, -7 and -9. Increased levels of caspase-3 are involved in Huntington Disease-associated cell death. Caspase-3 is the principal caspase in mediating the cleavage of amyloid-beta 4A precursor protein (APP), which is related with neuronal death in Alzheimer's disease. Like other caspases, caspase-3 is also synthesized as a zymogen procaspase which is activated by specific proteolytic cleavage. High levels of caspase-3 are observed in lung, spleen, heart, liver and kidney, moderate levels in brain and skeletal muscle, and low in testis.

549806822

NP_001271338.1

NM_001284409.1

P70677

Activation Of DNA Fragmentation Factor Pathway (1167770); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (1167760); Alpha6-Beta4 Integrin Signaling Pathway (198373); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198319)

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. Protein type: EC 3.4.22.56; Motility/polarity/chemotaxis; Apoptosis; Protease. Cellular Component: cytoplasm; cytosol; nucleus; lipid raft. Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; phospholipase A2 activator activity; protein binding; protease binding; hydrolase activity; cysteine-type endopeptidase activity; death receptor binding; protein complex binding; aspartic-type endopeptidase activity; cysteine-type peptidase activity. Biological Process: positive regulation of catalytic activity; apoptosis; positive regulation of apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; proteolysis; negative regulation of cell cycle; neuron differentiation; learning and/or memory; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; response to glucose stimulus; erythrocyte differentiation; T cell homeostasis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; response to hydrogen peroxide; protein processing; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis

0.1 mg

290 USD