Monoclonal Antibody to mTLR3 (ABM24E5) | MyBioSource MBS668027 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to mTLR3 (ABM24E5)

catalog :

MBS668027

quantity :

0.1 mg

price :

290 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[ABM24E5]

reactivity :

human, mouse

application :

western blot, immunohistochemistry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Intracellular flow analysis of mTLR3 in m Splenocytes using 0.5 tJg/1 0"6 cells of mTLR3 antibody (Clone: ABM24E5). Green represents isotype control; red represents anti-mTLR3 antibody. Goat anti-Rat FITe conjugate was used as secondary antibody .

image 2 :

Immunohistochemical analysis of mTLR3 in human Liver tissue using mTLR3 antibody (Clone:ABM24ES) at 10 ug/ml.

product information

catalog number :

MBS668027

products type :

Antibody

products full name :

Monoclonal Antibody to mTLR3 (ABM24E5)

products short name :

[mTLR3]

products name syn :

[Tlr3]

other names :

[toll-like receptor 3; Toll-like receptor 3; toll-like receptor 3; toll-like receptor 3; CD_antigen: CD283]

products gene name :

[mTLR3]

other gene names :

[Tlr3; Tlr3; AI957183]

uniprot entry name :

TLR3_MOUSE

clonality :

Monoclonal

isotype :

Rat IgG2b, Kappa

clone :

[ABM24E5]

host :

Rat

reactivity :

Mouse, Human

sequence length :

905

purity :

Protein G Chromatography

form :

Purified (Liquid)

storage stability :

Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.

tested application :

Western Blot (WB), FACS, Immunohistochemistry (IHC)

app notes :

Western blot analysis: 2-4 ug/ml; FACS analysis: 0.5 ug/10^6 cells; Immunohistochemical analysis: 10ug/ml

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry

image3 heading :

Immunohistochemical

other info1 :

Antigen: mTLR3. Content: 100 ug in 200ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

other info2 :

Immunogen: A partial length recombinant mTLR3 protein (Amino acid sequence 180-385) was used as the immunogen for this antibody.

products categories :

TLR

products description :

Mouse Toll-like receptor 3 (mTLR3) belongs to a family of evolutionary conserved innate immune recognition molecules and recognizes double-stranded RNA, a molecular pattern associated with viral infections. It has seven exons. mTlr3 show high homology to hTLR3 in their predicted cDNA and protein sequences. The intracellular region of mTlr3 has remarkable homology with the same region of others mTlrs. mTlr3 mRNA is ubiquitously expressed in tissues, being expressed highest in spleen, kidney and lung. Its activation by the synthetic ligand polyinosine:polycytidylic acid (poly I:C) or by mRNA rapidly causes growth cone collapse and irreversibly inhibits neurite extension independent of NF-kappaB. The high levels of mTlr3 expression in Mz B cells perhaps contribute to their ability to clear antigen from the blood.

ncbi gi num :

31543872

ncbi acc num :

NP_569054.2

ncbi gb acc num :

NM_126166.4

uniprot acc num :

Q99MB1

ncbi pathways :

Activated TLR4 Signalling Pathway (1168911); Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway (1168897); Apoptosis Pathway (1167740); Caspase Activation Via Extrinsic Apoptotic Signalig Pathway (1167741); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1168940); Hepatitis B Pathway (694729); Hepatitis C Pathway (173974); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865)

uniprot summary :

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon. Protein type: Receptor, cytokine; Membrane protein, integral. Cellular Component: cell surface; membrane; endoplasmic reticulum; lysosomal membrane; cytoplasm; integral to membrane; intracellular; endosome. Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; RNA binding; double-stranded RNA binding; receptor activity. Biological Process: positive regulation of interleukin-12 production; positive regulation of JNK cascade; microglial cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; defense response; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; inflammatory response; defense response to virus; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; response to virus; MyD88-independent toll-like receptor signaling pathway; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; microglial cell activation during immune response; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response

size1 :

0.1 mg

price1 :

290 USD

more info or order :

MyBioSource product webpage