ATF6 Monoclonal Antibody | MyBioSource MBS668021 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATF6 Monoclonal Antibody

catalog :

MBS668021

quantity :

0.1 mg

price :

290 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Expression analysis of ATF6. Anti- ATF6 antibody (Clone: ABM1A40) was used at 2 ug/ml on K562 lysate.

image 2 :

Intracellular staining of full length ATF6 plasmid and blank plasmid transfected HEK293 cells. 0.5 ug of anti-ATF6 antibody was used. Green represents blank plasmid, Red represents ATF6 expression plasmid. Goat anti-mouse PE was used as secondary antibody.

image 3 :

Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 ug/ml on (1) Blank plasmid transfected HEK293 cells, (2) Partial length ATF6 plasmid transfected HEK293 cells and (3) Fulll length ATF6 plasmid transfected HEK293 cells.

product information

catalog number :

MBS668021

products type :

Antibody

products full name :

ATF6 Monoclonal Antibody

products short name :

[ATF6]

other names :

[cyclic AMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; activating transcription factor 6; Activating transcription factor 6 alpha; ATF6-alpha]

products gene name :

[ATF6]

other gene names :

[ATF6; ATF6; ATF6A; cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha]

uniprot entry name :

ATF6A_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgG1, Kappa

host :

Mouse

reactivity :

Human

sequence length :

670

purity :

Protein G Chromatography

form :

Purified . 25 ug in 50 uL PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

storage stability :

Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

tested application :

FACS, WB

app notes :

Western Blot analysis: 2-4 ug/mL. FACS: 0.5 ug/10 6 cells

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry

image3 heading :

Western Blot

other info2 :

Immunogen: A partial recombinant ATF6 protein (amino acids 1-400) was used as the immunogen for this antibody

products categories :

Apoptosis

products description :

ATF6, also named as Cyclic AMP-dependent transcription factor ATF-6 alpha is a member of bZIP family, ATF subfamily. ATF6 forms homodimer and heterodimer with ATF6-beta. ATF6 acts by activating specific unfolded protein response target genes during endoplasmic reticulum stress. ATF6 is a ubiquitously expressed transcription factor located at endoplasmic reticulum (ER) membrane. During ER stress an approximately 50kDa fragment containing the N-terminal cytoplasmic domain is cleaved by proteolysis and is translocated into the nucleus. N-glycosylation may serve as a sensor for ER homeostasis. For the binding of ATF6 to ERSE, ESRE should first bind to another transcription factor called Nuclear Transcription Factor Y (NF-Y). ATF6 could also be involved in activation of transcription by interacting with serum response factor. ATF6 is a 74 kDa protein and is highly expressed in kidney, liver, colon and mammary gland.

ncbi gi num :

56786157

ncbi acc num :

NP_031374.2

ncbi gb acc num :

NM_007348.3

uniprot acc num :

P18850

ncbi pathways :

ATF4 Activates Genes Pathway (530772); ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Metabolism Of Proteins Pathway (106230); PERK Regulates Gene Expression Pathway (105907); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

uniprot summary :

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5 -CCAC[GA]-3 half of the ER stress response element (ERSE) (5 -CCAAT-N(9)-CCAC[GA]-3 ) and of ERSE II (5 -ATTGG-N-CCACG-3 ). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily. Protein type: Transcription factor; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus. Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; protein folding; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction. Disease: Achromatopsia 7

size1 :

0.1 mg

price1 :

290 USD

more info or order :

MyBioSource product webpage