product summary
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company name :
MyBioSource
product type :
protein
product name :
CD252(OX40L)-muCD8-Biotin
catalog :
MBS666846
quantity :
0.025 mg
price :
575 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS666846
products type :
Protein
products full name :
CD252(OX40L)-muCD8-Biotin
products short name :
[CD252]
products name syn :
[Human CD252(CD134L)-muCD8/Biotin]
products gene name :
[CD252]
host :
CHO cells
reactivity :
Human
form :
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
concentration :
0.5 mg/ml
storage stability :
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
tested application :
Flow Cytometry (FC/FACS), ELISA (EIA)
image1 heading :
Testing Data
other info1 :
Molecular Structure: A soluble molecule consisting of the extracellular domain (132 aa) of human CD252 fused to the extracellular domain (167aa) of murine CD8 alpha. Transfectant Cell Line: CHO. Performance: Five x 10 5 cultured human HPB-MLT cells per tube were washed and incubated 45 minutes on ice with 80 ul of CD252-muCD8/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log 10 fluorescent units when compared to a buffer control. Binding was blocked when reagent was pre incubated with anti-CD252 antibody at a concentration of 100 ug/ml.
other info2 :
Conjugation: Biotin. Production: Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column. Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
products description :
Information: Human CD252 (CD134L, OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 is involved with costimulatory activity between B cells and T cells (1). CD252-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.
products references :
1. W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2. L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3. A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.
size1 :
0.025 mg
price1 :
575 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
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