CD274 (B7-H1)-muIg-Biotin | MyBioSource MBS666818 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

CD274 (B7-H1)-muIg-Biotin

catalog :

MBS666818

quantity :

0.025 mg

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS666818

products type :

Protein

products full name :

CD274 (B7-H1)-muIg-Biotin

products short name :

[CD274]

products name syn :

[Human CD274(B7-H1)-muIg/Biotin Fusion Protein]

other names :

[CD274, partial; Programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; CD274 molecule; B7 homolog 1; B7-H1; CD_antigen: CD274]

products gene name :

[CD274]

other gene names :

[CD274; CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1]

uniprot entry name :

PD1L1_HUMAN

host :

CHO cells

reactivity :

Human

sequence length :

127

sequence :

Linker + Murine IgG2a Hinge + Fc (235aa):. (237)gteprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpg(471)
(13)ftvtvpkdlyvveygsnmtieckfpvekqldlaal
ivywemedkniiqfvhgeedlkvqhssyrqrarllkdql
slgnaalqitdvklqdagvyrcmisyggadykritvkvn
apynkinqrilvvdvtseheltcqaegypkaeviwtssd
hqvlsgkttttnskreeklfnvtstlrintttneifyct
frrldpeenhtaelvipelplahppnerthtr.
MuCD8 alpha signal peptide residual amino acids + linker:(1) kpqapelrgsas. CD274 mature EC: (224aa):

form :

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

concentration :

0.5 mg/ml

storage stability :

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Product should retain activity for at least 6 months after shipping date when stored as recommended.

tested application :

ELISA (EIA)

other info1 :

Molecular Structure: A soluble fusion protein consisting of the murine CD8 alpha leader sequence, the mature extracellular (224aa) domain of human CD274 fused to murine IgG2a Fc + hinge (233aa). Transfectant Cell Line: CHO. Performance: Purity of recombinant protein was >90% by SDS-PAGE. N-terminal sequence was as predicted: KPQAP. Biotinylated recombinant protein was active in EIA, binding to anti-CD274 mAbs ANC6H1, clone M1H1, and recombinant CD279(PD1), using SA/HRP for detection. Predicted monomeric (non glycosylated) molecular weight: 54.4kd. The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.

other info2 :

Conjugation: Biotin. Production: Human CD274-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column. Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). Molecular Weight: Predicted monomeric (non glycosylated) molecular weight: 54.4kd. The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions. Buffer: 50mM Sodium Phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

products description :

Information: CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection (3). CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma are associated with poor prognosis (1). Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival (2). Gamma Interferon and PHA can up regulate CD274 expression on T cells.

products references :

1)Cancer Research (April 2006) 66:3381. 2)J Molecular Medicine (Feb 2004) 81(5):281. 3)Int J Hematol (Nov 2003) 78(4):321.

ncbi gi num :

112363645

ncbi acc num :

ABI16084.1

ncbi pathways :

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552); Immune System Pathway (106386); PD-1 Signaling Pathway (119557)

uniprot summary :

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9p24. Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane. Molecular Function: protein binding. Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction

size1 :

0.025 mg

price1 :

575 USD

more info or order :

MyBioSource product webpage