protein

CD86-muIg-Biotin

MBS666812

0.025 mg

575 USD

Protein

CD86-muIg-Biotin

[CD86]

[Human CD86-muIg Fusion Protein]

[CD86, partial; T-lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 molecule; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD_antigen: CD86]

[CD86]

[CD86; CD86; B70; B7-2; B7.2; LAB72; CD28LG2; CD28LG2]

CD86_HUMAN

323

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

0.5 mg/ml

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Product should retain activity for at least 12 months after shipping date when stored as recommended.

Flow Cytometry (FC/FACS), ELISA (EIA)

Molecular Structure: A soluble fusion protein consisting of the extracellular (224aa) domain of human CD86 fused to murine IgG2a Fc (232aa). Predicted monomeric molecular weight 52.2 kd. Transfectant Cell Line: CHO

Production: Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column. Performance: CD86-muIg/Biotin was highly reactive at 5 ng/ml in an Enzyme Immuno Assay utilizing immobilized anti-CD86 antibody (Catalog #MBS666200) for capture, followed by Streptavidin/HRP and TMB/H 2 O 2 substrate chromagen for detection. It was also active at 50-100 ng/ml in a similar EIA utilizing immobilized recombinant CD152-muIg (Cat # MBS666792) for capture. Purified CD86-muIg blocked binding of CD152-muIg to CD86 on Raji cells in FACS. Five x 10 5 Raji cell per tube were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD80 mAb (Catalog #MBS666674) so that only CD86 binding would be apparent. They were then incubated 45 minutes on ice with 80 ul of CD152-muIg/R-PE (Catalog# MBS666880) at a concentration of 0.2 ug/ml, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.83Log 10 fluorescent units when compared to a Mouse IgG2a isotype control (Catalog #MBS666441) at a similar concentration. Binding was 60% blocked when the CD152-muIg/R-PE reagent was pre incubated with 50 ug/ml of CD86-muIg.

Information: Human CD86 (B7-2) is a costimulating ligand for CD28 and CTLA-4. CD86 is expressed on activated B cells and blood monocytes(3).

1. C. Caux, et al, (1994) J Exp Med 180: 1841-1847. 2. C.B. Thompson, (1995) Cell 81: 979-982. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 703-705. 4. D. Mauri, et al, (1995) J Immunol 155: 118-127.

49456643

CAG46642.1

P42081

28,420 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); CTLA4 Inhibitory Signaling Pathway (119556)

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 3q21. Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; coreceptor activity; receptor activity; receptor binding. Biological Process: positive regulation of lymphotoxin A biosynthetic process; nerve growth factor receptor signaling pathway; T cell activation; negative regulation of T cell anergy; viral reproduction; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; response to yeast; defense response to virus; aging; epidermal growth factor receptor signaling pathway; response to drug; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response

0.025 mg

575 USD