product summary
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company name :
MyBioSource
product type :
antibody
product name :
anti-human CD106-R-PE
catalog :
MBS666601
quantity :
120 Tests
price :
405 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
[1.G11B1]
application :
western blot, ELISA, immunohistochemistry, neutralization, flow cytometry, FACS, enzyme immunoassay
more info or order :
image
image 1 :
product information
catalog number :
MBS666601
products type :
Antibody
products full name :
anti-human CD106-R-PE
products short name :
[CD106]
products name syn :
[Monoclonal anti-human CD106(VCAM-1)/R-PE]
products gene name :
[CD106]
clonality :
Monoclonal
isotype :
IgG1
clone :
[1.G11B1]
host :
Mouse
reactivity :
Human, Pig
storage stability :
Store at 2 - 5 degree C. Do not freeze! Protect from light. Product should retain activity for at least 12 months after shipping date when stored as recommended.
tested application :
Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Blocking
app notes :
Dilution: 1:50 (or use 1.6ul of concentrated reagent per 5x10 5 -cell test)
image1 heading :
Testing Data
other info1 :
Performance: Cultured human umbilical cord vein endothelial cells were stimulated 4 hours with 10 ng/ml Phorbol 12-Myristate 13-Acetate. Five x 10 5 cells per tube were harvested, washed and incubated 45 minutes on ice with 80 ul of anti-CD106/R-PE at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.36 log 10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD106 antibody.
other info2 :
Conjugation: PE. Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A 565 /A 280 ratio of 3.53. Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). Immunogen: Human endothelial cells
products description :
Information: Human CD106 is an endothelial adhesion molecule that binds to alpha 4 beta 1 & alpha 4 beta 7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells. Antibody 1.G11B1 recognizes the VCAM-1 molecule (CD106) and blocks leukocyte adhesion.
products references :
M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767. M. Nagata, et al, (1995) J Immunol 155: 2194-2202. H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-314.
size1 :
120 Tests
price1 :
405 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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