antibody

anti-human CD252(OX40L)-Purified Preservative Free

MBS666578

0.1 mg

310 USD

monoclonal

mouse

nonconjugated

[ANC10G1]

ELISA, neutralization, flow cytometry, FACS, enzyme immunoassay

Antibody

anti-human CD252(OX40L)-Purified Preservative Free

[CD252]

[Monoclonal anti-human CD252 (CD134L, OX40L)]

[CD252]

Monoclonal

IgG1k

[ANC10G1]

Mouse

Human

Purified Preservative Free

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl

1.0 mg/ml

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions. Product should retain activity for at least 12 months after shipping date when stored as recommended.

Flow Cytometry (FC/FACS), ELISA (EIA), Blocking

Testing Data

Performance: Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD134L antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 14% subpopulation of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions. Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Immunogen: Human HUVEC and Recombinant soluble human CD134L

Information: Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of inflammation and rejection (5,6,7,8). Antibody from clone ANC10G1 binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.

1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2) L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. 4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 5) V. Malmstrom, et al, (2001) J Immunol 166: 6972. 6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115. 7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955. 8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.

0.1 mg

310 USD