antibody

anti-human CD357(GITR)-Purified (with Antibiotics)

MBS666296

0.1 mg

310 USD

monoclonal

mouse

nonconjugated

ANC5E3

ELISA, flow cytometry, FACS, enzyme immunoassay

Antibody

anti-human CD357(GITR)-Purified (with Antibiotics)

CD357

Monoclonal anti-human CD357 (GITR AITR)

CD357

Monoclonal

IgG3k

ANC5E3

Mouse

Human

Purified (with Antibiotics)

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate

1.0 mg/ml

Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Product should retain activity for at least 12 months after shipping date when stored as recommended.

Flow Cytometry (FC/FACS), ELISA (EIA)

Performance: Ficoll prepared human peripheral blood leukocytes (PBL) were stimulated 5 days in culture with PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-GITR mAb at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat-anti-Mouse Ig/FITC, after which they were washed three times and analyzed by FACS. Cells stained positive with a mean shift of 0.52 log10 fluorescent units when compared to a Mouse IgG3 negative control at a similar concentration.

Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin. Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative). Group Header: anti-human CD357(GITR). Immunogen: Recombinant human GITR, PHA stimulated human PBL

Information: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in anti-parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3). Clone ANC5A3 binds to human GITR in EIA and on stimulated cell surface in FACS. Preincubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.

1) Gurney AL, et al. (1999) Curr Biol 9(4): 215-218. 2) Ji HB, et al. (2004) J Immunol 172(10): 5823-5827. 3) Kim WJ, WH Lee, et al. (2006) Immunology 119(3): 44-9. 4) Furze RC, Selkirk ME, et al. (2006) Microbes Infect 8(12-13): 2803-10. 5) Kohm AP, SD Miller, et al. (2004) J Immunol 172: 4686-4690. 6) McHugh, R.S. et al. (2002) Immunity 16:311. 7) Shin, H.H. et al. (2002) FEBS Lett. 514:275. 8). Shimizu, J. et al. (2002) Nature Immunol. 3:135.

0.1 mg

310 USD