product summary
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company name :
MyBioSource
product type :
antibody
product name :
anti-human CD62L-APC
catalog :
MBS666271
quantity :
120 Tests
price :
470 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
LAM1-116
application :
flow cytometry, FACS
more info or order :
product information
catalog number :
MBS666271
products type :
Antibody
products full name :
anti-human CD62L-APC
products short name :
CD62L
products name syn :
Monoclonal anti-human CD62L (L-selectin)/APC
products gene name :
CD62L
clonality :
Monoclonal
isotype :
IgG2ak
clone :
LAM1-116
host :
Mouse
reactivity :
Human, Horse, Mouse, Guinea Pig, Rat, Dog, Cow
concentration :
Volume: 0.2 ml
storage stability :
Store at 2 - 5 degree C. Do not freeze! Protect from light. Product should retain activity for at least 12 months after shipping date when stored as recommended.
tested application :
Flow Cytometry (FC/FACS)
app notes :
1:50 (or use 1.6ul of concentrated stock per 5 x 10 5 cell test)
other info2 :
Conjugation: APC. Production: Protein A purified antibody from tissue culture supernatant was conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A 650 /A 280 ration of 2.21. Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). Immunogen: Mouse pre-B cells expressing human L-selectin. Performance: Five x 10^5 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD62L/APC at a 1:50 dilution factor (10ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.1 log 10 fluorescent units when compared to a Mouse IgG2a/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD62L antibody.
products description :
Information: Human CD62L is as adhesion molecule found on most leukocytes and plays a primary role in mediating initial leukocyte interaction with activated vascular endothelium (1). Human CD62L has signal transduction functions where engagement leads to increased calcium levels and upregulated expression of certain integrins (2). Antibody LAM1-116 recognizes the lectin binding domain of CD62L (2). Antibody LAM1-116 blocks CD62L function and induces expression of beta-1 and beta-2 integrins (1).
products references :
(1). T.A. Springer, (1995) Annu Rev Physiol 57: 827-872. (2). D.A. Steeber, et al, (1997) J Immunol 159: 952-963. (3). Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997)
p. 420-424.
size1 :
120 Tests
price1 :
470 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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