antibody

anti-human CD137L-Purified Preservative Free

MBS666242

0.1 mg

310 USD

monoclonal

mouse

nonconjugated

[ANC5D6]

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

Antibody

anti-human CD137L-Purified Preservative Free

[CD137L]

[Monoclonal anti-human CD137 L (4-1BB Ligand)]

[CD137L]

Monoclonal

Murine IgG2a, kappa

[ANC5D6]

Mouse

Human

Purified Preservative Free

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl

1.0 mg/ml

Store at 2 - 5°C. Open under aseptic conditions. Freeze/Thawing is not recommended. Product should retain activity for at least 6 months after shipping date when stored as recommended

Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)

Testing Data

Performance: Five x 10^5 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 ul of anti-CD137L antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, MBS666783 after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.29 log 10 fluorescent units when compared to a Mouse IgG2a negative control MBS666112 at a similar concentration.

Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions. Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Immunogen: Recombinant human CD137L

Information: Human CD137 Ligand is a type II transmembrane protein constitutively expressed by monocytes, B cells neuroblastoma cells (1), and some activated T cells. The interaction of CD137 (4-1BB) and CD137L can supply a costimulatory signal to T cells through CD137. This costimulus seems to be more effective for CD8+ effector T cells than for CD4+ helper T cells (3). The interaction can also effect a reverse signal, inducing monocyte activation (2), or T cell inactivation and programmed death (4) through CD137L. Anti-CD137L antibody binds to CD137L present on Raji and U-937 cells, and also binds to recombinant CD137L-muCD8 in EIA. This antibody does not block the interaction between CD137 and CD137L.

1) M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227. 2) J. Langstein, et al, (1998) J Immunol 160: 2488-2494. 3) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 4) H. Schwartz, et al, (1996) Blood 87: 2839. 5) G. Zhu, et al, (2001) J Immunol 167: 2671-6.

0.1 mg

310 USD