antibody

Monoclonal mouse anti-human MUC1 glycoprotein

MBS660081

1 mg

505 USD

monoclonal

mouse

nonconjugated

CM1, M9E7, M4H2, M8C9, M10G4, M10H6, M2F1, M2C5, M

ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Monoclonal mouse anti-human MUC1 glycoprotein

Mucin-1 Glycoprotein

Mucin-1; Mucin-1; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant; mucin 1, cell surface associated; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT

MUC1

MUC1; MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta

MUC1_HUMAN

Monoclonal

IgG for MAbs M9E7, M4H2, M8C9, M10G4, M10H6, M2F1, M2C5, M2E104 and M3A106. IgG1 for MAb CM1

CM1, M9E7, M4H2, M8C9, M10G4, M10H6, M2F1, M2C5, M2E104, M3A106

mouse

1255

MAb CM1: peptide tandem repeat in MUC1 core. Similar to DF3 MAb (Centdegree Cor). MAbs M9E7, M8C9, M10G4, M10H6:. MAbs bind with high efficiency with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. They react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These MAbs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific MAbs and belong to MAb cluster 1-4 according to ISOMB TD-4 classification. No cross-reactivity with egg white avidin. MAbs M4H2, M2F1, M2C5, M2E104 and M3A106:. MAbs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. MAb M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (and very weakly with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1 (but not with completely glycosylated natural milk MUC1). MAbs M2F1, M2C5, M2E104, M3A106 efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. All these MAbs belong to MAb clusters 6 and 7 according to ISOMB TD-4 classification. Non crossreactive with normal serum proteins and human tissues of non-epithelial origin.

Chromatography on protein A Sepharose

PBS, pH 7.4, 0.1 % sodium azide (NaN3)

4 degree C

Immunohistdegree Chemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

Immunohistdegree Chemical and immunofluorescent detection of neoplastic breast epithelium. Antibodies are working on frozen tissue sections. Construction of immunometric assay for MUC determination. MAbs M9E7, M4H2, M8C9, M10G4, M10H6 can be used for construction of highly effective ELISA tests for detection and measurements of unglycosylated and underglycosylated tumor-assdegree Ciated MUC1 mucins in human serum. They may be used as solid-phase reagents for MUC1 isoforms capture. (a) in conjunction with the same MAbs enzyme-conjugated versions (detection of unglycosylated MUC1), or. (b) with enzyme-conjugated MAbs M2C5, M2E10 or M3A10 specific to carbohydrate portions of MUC1 (detection of underglycosylated MUC1). MAbs M4H2, M2F1, M2C5, M2E104, M3A106 can be used for immunohistdegree Chemical staining of paraffin and frozen sections of breast, lung and ovarian tumor tissues. They may be used for staining of paraffin-embedded tissue sections fixed in neutral-buffered formalin and is compatible with commonly used histological fixatives. Revealed MUC1 epitopes are abundantly present as on the surface of growing tumor cell lines, so as on the surface and into the cytoplasm of malignant cells of epithelial origin. These MAbs can be used in the form of enzyme conjugates for development in ELISA sandwich assays of under-glycosylated tumor-assdegree Ciated MUC1 mucins in human serum. They may be used in conjunction with MAbs M9E7, M4H2, M8C9, M10G4 and M10H6 specific to unglycosylated VNTR region of MUC1 as capture reagents.

Clone Note: Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with high molecular weight (>300 kDa) glycoprotein from human milk-fat globule molecule

296439295

P15941.3

28,297 Da

IL-7 Signaling Pathway 198857!!Metabolism Of Proteins Pathway 106230!!O-linked Glycosylation Pathway 1127679!!O-linked Glycosylation Of Mucins Pathway 530747!!Post-translational Protein Modification Pathway 161012!!Termination Of O-glycan Biosynthesis Pathway 530748

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

1 mg

505 USD