protein

FOXO4 (Forkhead Box O4, AFX1) Control Peptide (KLH)

MBS658857

1 mg

310 USD

Peptide

FOXO4 (Forkhead Box O4, AFX1) Control Peptide (KLH)

[FOXO4]

[FOXO4 (Forkhead Box O4, AFX1) Control Peptide (KLH)]

[forkhead box protein O4 isoform 1; Forkhead box protein O4; forkhead box protein O4; fork head domain transcription factor AFX1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; forkhead box O4; Fork head domain transcription factor AFX1]

[FOXO4; FOXO4; AFX; AFX1; MLLT7; AFX; AFX1; MLLT7]

FOXO4_HUMAN

95.89%

Supplied as a white to off-white powder. Conjugates to KLH.

Lyophilized powder may be stored at 4°C for short-term only. Stable for 6 months after receipt at -20°C . Reconstitute(see reconstitution for peptides)and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry and or Immunocytochemistry.

Source: Synthetic. Amino Acid Sequence (linear): Met-Asp-Pro-Gly-Asn-Glu-Asn-Ser-Ala-Thr-Glu-Ala-Ala-Ala-lle-KLH. Molecular Formula: C 59 H 95 N 17 O 26 S. Molecular Weight: 1490.68

Molecular Biology; MB-Transcription Factors

103472003

NP_005929.2

NM_005938.3

P98177

AGE/RAGE Pathway (698754); AKT Phosphorylates Targets In The Nucleus Pathway (106476); Adaptive Immune System Pathway (366160); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signal Transduction Pathway (106385)

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Function: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Ref.8 Ref.10 Ref.11 Ref.12 Ref.14 Ref.17 Ref.19 Ref.20. Subunit structure: Interacts with CREBBP/CBP, CTNNB1, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CREBBP/CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Interacts with USP7; the interaction is enhanced in presence of hydrogen peroxide and occurs independently of TP53. Interacts with NLK, and this inhibits monoubiquitination and transcriptional activity. Ref.12 Ref.13 Ref.14 Ref.17 Ref.19. Subcellular location: Cytoplasm. Nucleus. Note: When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. Ref.9 Ref.13 Ref.17. Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. Post-translational modification: Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Ref.8 Ref.9 Ref.15 Ref.18Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. Involvement in disease: A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with KMT2A/MLL1. The result is a rogue activator protein. Pharmaceutical use: A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-197 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity. Sequence similarities: Contains 1 fork-head DNA-binding domain.

1 mg

310 USD