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RNA Marker (Ladder), High Range

MBS657630

0.02 mL

290 USD

Marker

RNA Marker (Ladder), High Range

[RNA Marker]

Supplied as a liquid in 1mM EDTA (pH 6.0)

Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

2X Loading Dye Solution: Supplied as a liquid in 95% formamide, 0.025% SDS, 0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide, 0.5mM EDTA.

Quality Control: Well defined bands have been formed when a 2 ul aliquot of RNA Ladder, High Range was premixed with the 2X RNA Loading Dye Solution and on a native agarose gel. Note: The ATP concentration should be at least 1 uM. T4 Polynucleotide kinase is inhibited by trace level of phosphate or ammonium salts. Caution: RNA ladders, as any RNA are extremely sensistive to degradation by ribonucleases. To avoid RNA degradation, protective gloves should be used and electrophoresis buffers and gels should be freshly prepared. Plasticwatre tips and solutions hsould be treated with diethylpyrocarbonate. Gloves are also necessary to handle solutions containing ethidium bromide.

Cloning; Cloning-RNA Markers

The RNA Ladder, High Range, is a set of eight RNA transcripts with lengths of 6000, 4000, 3000, 2000, 1500,1000, 500 and 200 bases. RNA transcripts are produced from specific templates, containing a fragment of the Ptz19r polylinker and lambda phage sequences. The RNA Ladder, High Range, is suitable for analysis of RNAs in native or denaturing agarose or polyacrylamide gels and can be easily visualized by ethidium bromide staining.

0.02 mL

290 USD

0.1 mL

575