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Bence Jones kappa Protein, Human

MBS655090

1 mg

705 USD

Immunoglobulin

Bence Jones kappa Protein, Human

[Bence Jones kappa Protein]

Human urine

>98% Purified. Purified by chromatography.

Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 500ul sterile ddH2O. Dilute further with PBS.

~2mg/ml(after reconstitution)

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C . Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product centrifuge the original vial after thawing and prior to removing the cap.

Suitable for use as a reference antigen, calibrator, coating protein and blocking agent in Immunoelectrophoresis, Double RID, ELISA, and Western Blot. Other applications have not been tested.

Optimal dilutions to be determined by the researcher.

Molecular Biology; MB-Immunoglobulins

A Bence Jones protein is a monoclonal globulin protein or immunoglobulin light chain found in the urine, with a molecular weight of 22-24 kD. Detection of Bence Jones protein may be suggestive of multiple myeloma or Waldenstrom’s macroglobulinemia. Bence Jones proteins are particularly diagnostic of multiple myeloma in the context of end-organ manifestations such as renal failure, lytic( or’’ punched out’’) bone lesions , anemia or large numbers of plasma cells in the bone marrow of patients. Bence Jones proteins are present in 2/3 of multiple myeloma cases. The proteins are immunogloblulin light chains(paraproteins) and are produced by neoplastic plasma cells. They can be kappa( most of the time) of lambda. The light chains can be immunoglobulin fragments or single homogeneous immunoglobulins. They are found in urine as a result of decreases kidney filtration capabilities due to renal failure, sometimes induced by hypercalcemia from the calcium released as the bones are destroyed or form the light chains themselves. (citation needed) The light chains have historically been detected by heating a urine specimen ( which causes the protein to precipitate) and now by electrophoresis of concentrated urine. More recently,serum free light chain assays have been utilized in a number of published studies which have been indicated superiority over the urine tests, particularly for patients producing low levels of monoclonal free light chains, as seen in monsecretory multiple myeloma and AL amyloidosis. This is primarily because of the re-absorption of free light chains in the kidneys, creating a threshold of light chains production which must be exceeded before measurable quantities overflow into the urine. As such urinalysis is a fickle witness to changing free light chain production.

1 mg

705 USD