catalog number :
MBS654007
products full name :
Uricase, Candida utilis
products short name :
[Uricase, Candida utilis]
purity :
Chromatographically purified
form :
Supplied as a lyophilized powder
storage stability :
-20 degree C
image1 heading :
Testing Data
other info1 :
Source: Candida utilis. Quality Control: SDS-PAGE. Activity: 4.41 units/mg. Unit Definition: One unit oxidizes on micromole of uric acid per minute at 25 degree C, pH 8.5. Composition: The enzyme is composed of four subunits of 32,000 MW. There is one copper atom per molecule (125,000 MW). Optimum pH: 9.0. Extinction Coefficient: 11.3. Isoelectric Point: 6.3. A260(mg/ml): 0.320. A280 (mg/ml): 0.530. Inhibitors: Various purine analog of urate, cyanide and otehr copper chelating agents. Urate in alkaline solution may be converted to a potent inhibitor, oxonate.
products categories :
Molecular Biology; MB-Enzymes
products description :
Uricase is very important for the determination of uric acid in biological fluids. Not only is the reaction specific, it may be monitored at 292nm, 340nm or colorimetrically by coupled chromogenic response. Non-enzymatic methods are interfered with by turbidity or the presence of aspirin, ascorbic acid, glutathione, paracetanol and many antibiotics. Uricase (urate oxidase) catalyzes the overall reaction that represents the termination of purine catabolism in all mammals excluding man, higher apes and the Dalmatian dog.
ncbi acc num :
BAA06804.1
uniprot summary :
Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.